A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs

被引：278

作者：

Huang, Chuan ^{[1
]}

Liang, Dongming ^{[1
]}

Tatomer, Deirdre C. ^{[1
]}

Wilusz, Jeremy E. ^{[1
]}

机构：

[1] Univ Penn, Dept Biochem & Biophys, Perelman Sch Med, Philadelphia, PA 19104 USA

来源：

GENES & DEVELOPMENT | 2018年 / 32卷 / 9-10期

基金：

美国国家卫生研究院;

关键词：

circRNA; Hel25E; UAP56; URH49; DDX39A; DDX39B; DEXH/D BOX PROTEIN; MESSENGER-RNA; EXPRESSION; UAP56; BIOGENESIS; HELICASES; CIRCRNA; BINDING; URH49;

D O I：

10.1101/gad.314856.118

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Circular RNAs (circRNAs) are generated from many protein-coding genes. Most accumulate in the cytoplasm, but how circRNA localization or nuclear export is controlled remains unclear. Using RNAi screening, we found that depletion of the Drosophila DExH/D-box helicase Hel25E results in nuclear accumulation of long (>800-nucleotide), but not short, circRNAs. The human homologs of Hel25E similarly regulate circRNA localization, as depletion of UAP56 (DDX39B) or URH49 (DDX39A) causes long and short circRNAs, respectively, to become enriched in the nucleus. These data suggest that the lengths of mature circRNAs are measured to dictate the mode of nuclear export.

引用

页码：639 / 644

页数：6

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