Rhizobium smilacinae sp nov., an endophytic bacterium isolated from the leaf of Smilacina japonica

被引：11
作者：
Zhang, Lei [1 ,2 ]
Shi, Xu [1 ,2 ]
Si, Meiru [1 ,2 ]
Li, Changfu [1 ,2 ]
Zhu, Lingfang [1 ,2 ]
Zhao, Liang [1 ,2 ]
Shen, Xihui [1 ,2 ]
Wang, Yao [1 ,2 ]
机构：
[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
[2] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China
来源：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2014年 / 106卷 / 04期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
Rhizobium smilacinae sp nov; Endophytic bacterium; Leaf of Smilacina japonica; CLOSE PHYLOGENETIC RELATIONSHIP; DEOXYRIBONUCLEIC-ACID; EMENDED DESCRIPTION; ROOT; CLASSIFICATION; RELATEDNESS; GENUS; STEM;
D O I：
10.1007/s10482-014-0241-1
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
During a study of endophytic bacteria from traditional Chinese medicinal plants, a bacterial strain, designated PTYR-5(T), was isolated from the leaf of Smilacina japonica A. Gray collected from Taibai Mountain in Shaanxi Province, north-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PTYR-5(T) is a member of the genus Rhizobium, exhibiting the highest sequence similarities to R. cellulosilyticum LMG 23642(T) (97.2 %), R. huautlense LMG 18254(T) (97.2 %) and R. alkalisoli CCBAU 01393(T) (97.1 %). The levels of 16S rRNA gene sequence similarity with respect to other Rhizobium species with validly published names were less than 97.0 %. Phylogenies of the housekeeping genes atpD, recA and glnII confirmed its distinct position, showing low similarity with respect to those of recognized Rhizobium species (no more than 94.1aEuroS, 90.0 and 88.0 aEuroS % similarity, respectively). The DNA-DNA relatedness values of strain PTYR-5(T) with R. cellulosilyticum LMG 23642(T), R. huautlense LMG 18254(T) and R. alkalisoli CCBAU 01393(T) were 33.6, 21.4 and 29.5 %, respectively. Based on phenotypic, phylogenetic and genotypic data, strain PTYR-5(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium smilacinae sp. nov. is proposed. The type strain is PTYR-5(T) (=CCTCC AB 2013016(T)=KCTC 32300(T)=LMG 27604(T)).
引用
收藏
页码：715 / 723
页数：9
相关论文
共 41 条
[1]   Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules [J].
Amarger, N ;
Macheret, V ;
Laguerre, G .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (04) :996-1006
[2]   Rhizobium halophytocola sp nov., isolated from the root of a coastal dune plant [J].
Bibi, Fehmida ;
Chung, Eu Jin ;
Khan, Ajmal ;
Jeon, Che Ok ;
Chung, Young Ryun .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012, 62 :1997-2003
[3]  
Cleenwerck I, 2002, INT J SYST EVOL MICR, V52, P1551, DOI 10.1099/00207713-52-5-1551
[4]  
DOETSCH R.N., 1981, MANUAL METHODS MICRO, P21, DOI DOI 10.1093/MOLBEV/MSR121
[5]  
Euzeby J.P., 2014, List of Prokaryotic Names with Standing in Nomeclature-Genus Staphylococcus
[6]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[7]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[8]  
Frank B., 1889, Bet Dtsch Bot Ges, V7, P332, DOI [10.1111/j.1438-8677.1889.tb05711.x, DOI 10.1111/J.1438-8677.1889.TB05711.X]
[9]   Evolutionary Dynamics of Nitrogen Fixation in the Legume-Rhizobia Symbiosis [J].
Fujita, Hironori ;
Aoki, Seishiro ;
Kawaguchi, Masayoshi .
PLOS ONE, 2014, 9 (04)
[10]   NUMERICAL TAXONOMY AND DNA RELATEDNESS OF TROPICAL RHIZOBIA ISOLATED FROM HAINAN PROVINCE, CHINA [J].
GAO, JL ;
SUN, JG ;
LI, Y ;
WANG, ET ;
CHEN, WX .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (01) :151-158
12345