Carotenoid composition of Rosa canina fruits determined by thin-layer chromatography and high-performance liquid chromatography

被引：62
作者：
Hodisan, T
Socaciu, C
Ropan, I
Neamtu, G
机构：
[1] UNIV AGR SCI & VET MED,DEPT CHEM & BIOCHEM,CLUJ NAPOCA 3400,ROMANIA
[2] UNIV BABES BOLYAI,DEPT ANALYT CHEM,R-3400 CLUJ NAPOCA,ROMANIA
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 16卷 / 03期
关键词：
Rosa canina (Rosaceae); carotenoids; thin-layer chromatography; high-performance liquid chromatography;
D O I：
10.1016/S0731-7085(97)00099-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The carotenoid composition of fruits of Rosa canina (Rosaceae) was determined comparatively by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) in total extracts and in three different fractions derived from previous separation of the total fruit extract on alumina columns. Both chromatographic analyses revealed as major carotenoids: beta-carotene, lycopene, beta-chryptoxanthin, rubixanthin, zeaxanthin and lutein. The distribution of these compounds was reproducible by TLC and by HPLC. The I-III fractions eluted successively from alumina columns by increasing the polarity of the solvents were analysed also by TLC and HPLC. In all situations, carotenoids were better separated and identified by gradient HPLC systems than by isocratic HPLC or TLC. (C) 1997 Elsevier Science B.V.
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页码：521 / 528
页数：8
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