Transplantation of bone marrow mesenchymal stem cells pretreated with valproic acid in rats with an acute spinal cord injury

被引：19
作者：
Chen, Lei [1 ,2 ]
Cui, Xiaoyan [1 ]
Wu, Zhourui [2 ]
Jia, Long [2 ]
Yu, Yan [2 ]
Zhou, Qiulian [3 ]
Hu, Xiao [2 ]
Xu, Wei [2 ]
Luo, Dandan [1 ]
Liu, Jie [1 ]
Xiao, Junjie [3 ]
Yan, Qiao [1 ]
Cheng, Liming [1 ,2 ]
机构：
[1] Tongji Univ, Sch Med, Tongji Hosp, Translat Ctr Stem Cell Res,Dept Regenerat Med, Shanghai 200065, Peoples R China
[2] Tongji Univ, Sch Med, Tongji Hosp, Dept Spinal Surg, Shanghai 200065, Peoples R China
[3] Shanghai Univ, Coll Life Sci, Shanghai, Peoples R China
来源：
BIOSCIENCE TRENDS | 2014年 / 8卷 / 02期
基金：
中国国家自然科学基金; 美国国家科学基金会;
关键词：
Mesenchymal stem cells derived from bone marrow; spinal cord injury; valproic acid; stromal derived factor-1; CXC chemokine receptor 4; STROMAL CELLS; IN-VITRO; MIGRATION; MODEL; MICE; DIFFERENTIATION; ANGIOGENESIS; PROMOTES; IMPROVES; LESION;
D O I：
10.5582/bst.8.111
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This study aimed to investigate whether valproic acid (VPA) pretreatment enhances the therapeutic effectiveness of mesenchymal stem cells derived from bone marrow (BMSCs) transplanted into rats with an acute spinal cord injury (SCI). BMSCs were pretreated with VPA before transplantation and then intravenously injected 1 week after SCI. Before transplantation, levels of CXC chemokine receptor 4 (CXCR4) expression in BMSCs were tested using quantitative real-time PCR and Western blotting. Stromal derived factor-1 (SDF-1), the unique ligand of CXCR4, was quantified using RT-PCR and immunofluorescence. The locomotor function of rats with an SCI was evaluated using the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Fluorescence microscopy and hematoxylin-eosin (HE) staining were also performed to evaluate pathophysiological changes after transplantation. On day 7 after SCI, the level of SDF-1 expression peaked. CXCR4 expression increased significantly in BMSCs pretreated with VPA. After intravenous transplantation, BrdU-labeled BMSCs were noted at the spinal injury site, and this was especially true for BMSCs pretreated with VPA. More significant functional improvement was observed in rats receiving BMSCs pretreated with VPA than in other groups of rats. AMD3100 partially inhibited improvement. This study demonstrates that pretreatment with VPA before transplantation enhances the therapeutic benefits of BMSCs in terms of greater cell migration and better neurological outcomes after traumatic SCI. The mechanism of this enhancement may be related to the SDF-1/CXCR4 axis. Therefore, pretreatment of BMSCs with VPA warrants further study in relation to the treatment of traumatic SCI.
引用
收藏
页码：111 / 119
页数：9
相关论文
共 31 条
[11]   Neuroprotective effects of granulocyte colony-stimulating factor and relationship to promotion of angiogenesis after spinal cord injury in rats Laboratory investigation [J].
Kawabe, Junko ;
Koda, Masao ;
Hashimoto, Masayuki ;
Fujiyoshi, Takayuki ;
Furuya, Takeo ;
Endo, Tomonori ;
Okawa, Akihiko ;
Yamazaki, Masashi .
JOURNAL OF NEUROSURGERY-SPINE, 2011, 15 (04) :414-421
[12]   Intravenous hMSCs Improve Myocardial Infarction in Mice because Cells Embolized in Lung Are Activated to Secrete the Anti-inflammatory Protein TSG-6 [J].
Lee, Ryang Hwa ;
Pulin, Andrey A. ;
Seo, Min Jeong ;
Kota, Daniel J. ;
Ylostalo, Joni ;
Larson, Benjamin L. ;
Semprun-Prieto, Laura ;
Delafontaine, Patrice ;
Prockop, Darwin J. .
CELL STEM CELL, 2009, 5 (01) :54-63
[13]   Effect of Neuroglobin Genetically Modified Bone Marrow Mesenchymal Stem Cells Transplantation on Spinal Cord Injury in Rabbits [J].
Lin, Wen-Ping ;
Chen, Xuan-Wei ;
Zhang, Li-Qun ;
Wu, Chao-Yang ;
Huang, Zi-Da ;
Lin, Jian-Hua .
PLOS ONE, 2013, 8 (05)
[14]   Improvement of contusive spinal cord injury in rats by co-transplantation of gamma-aminobutyric acid-ergic cells and bone marrow stromal cells [J].
Mohammad-Gharibani, Payam ;
Tiraihi, Taki ;
Delshad, Alireza ;
Arabkheradmand, Jalil ;
Taheri, Taher .
CYTOTHERAPY, 2013, 15 (09) :1073-1085
[15]   Transplantation of Mesenchymal Stem Cells Promotes an Alternative Pathway of Macrophage Activation and Functional Recovery after Spinal Cord Injury [J].
Nakajima, Hideaki ;
Uchida, Kenzo ;
Guerrero, Alexander Rodriguez ;
Watanabe, Shuji ;
Sugita, Daisuke ;
Takeura, Naoto ;
Yoshida, Ai ;
Long, Guang ;
Wright, Karina T. ;
Johnson, William E. B. ;
Baba, Hisatoshi .
JOURNAL OF NEUROTRAUMA, 2012, 29 (08) :1614-1625
[16]   Intravenous administration of mesenchymal stem cells derived from bone marrow after contusive spinal cord injury improves functional outcome [J].
Osaka, Misuzu ;
Honmou, Osamu ;
Murakami, Tomohiro ;
Nonaka, Tadashi ;
Houkin, Kiyohiro ;
Hamada, Hirofumi ;
Kocsis, Jeffery D. .
BRAIN RESEARCH, 2010, 1343 :226-235
[17]   Mesenchymal Stem Cell Graft Improves Recovery after Spinal Cord Injury in Adult Rats through Neurotrophic and Pro-Angiogenic Actions [J].
Quertainmont, Renaud ;
Cantinieaux, Dorothee ;
Botman, Olivier ;
Sid, Selim ;
Schoenen, Jean ;
Franzen, Rachelle .
PLOS ONE, 2012, 7 (06)
[18]   Adult bone marrow stromal cells differentiate into neural cells in vitro [J].
Sanchez-Ramos, J ;
Song, S ;
Cardozo-Pelaez, F ;
Hazzi, C ;
Stedeford, T ;
Willing, A ;
Freeman, TB ;
Saporta, S ;
Janssen, W ;
Patel, N ;
Cooper, DR ;
Sanberg, PR .
EXPERIMENTAL NEUROLOGY, 2000, 164 (02) :247-256
[19]   Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases [J].
Son, Bo-Ra ;
Marquez-Curtis, Leah A. ;
Kucia, Magda ;
Wysoczynski, Marcin ;
Turner, A. Robert ;
Ratajczak, Janina ;
Ratajczak, Mariusz Z. ;
Janowska-Wieczorek, Anna .
STEM CELLS, 2006, 24 (05) :1254-1264
[20]   Identification of Chemoattractive Factors Involved in the Migration of Bone Marrow-Derived Mesenchymal Stem Cells to Brain Lesions Caused by Prions [J].
Song, Chang-Hyun ;
Honmou, Osamu ;
Furuoka, Hidefumi ;
Horiuchi, Motohiro .
JOURNAL OF VIROLOGY, 2011, 85 (21) :11069-11078
1234