Forkhead box O1 in turbot Scophthalmus maximus: Molecular characterization, gene structure, tissue distribution and the role in glucose metabolism

被引：16
作者：
Pan, Mingzhu [1 ]
Zhang, Yue [1 ]
Deng, Kangyu [1 ]
Liu, Guangxia [1 ]
Gu, Zhixiang [1 ]
Liu, Jiahuan [1 ]
Luo, Kai [1 ]
Zhang, Wenbing [1 ,2 ]
Mai, Kangsen [1 ,2 ]
机构：
[1] Ocean Univ China, Minist Agr & Rural Affairs, Key Lab Aquaculture Nutr & Feeds, Key Lab Mariculture,Minist Educ, Qingdao 266003, Shandong, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Wen Hai Rd, Qingdao 266237, Shandong, Peoples R China
来源：
GENE | 2019年 / 708卷
基金：
中国国家自然科学基金;
关键词：
Turbot; Forkhead box O1; siRNA; Glucose; Metabolism; INSULIN-BINDING; FOXO1; EXPRESSION; FISH; GLUCONEOGENESIS; CARBOHYDRATE; STARCH; GROWTH; LIVER; DIETS;
D O I：
10.1016/j.gene.2019.03.065
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Forkhead box O1 (foxo1) is a transcription factor and plays important roles in glucose metabolism. In the present study, foxo1 in turbot Scophthalmus maximus was cloned and characterized. The siRNA of foxo1 was used to investigate the functions of foxo1 in turbot hepatocytes glucose metabolism. After that, a 10-week feeding trial with two different dietary carbohydrate levels (15% and 21%, respectively) was conducted to analyze the function of foxo1 in glucose metabolism in vivo. Results showed that the foxo1 was identified as 2176 bp (base pair) with a 2025 bp open reading frame, which encoded 675 amino acids. Sequence analysis showed that foxo1 of turbot was highly homologous to most of fishes. Tissue distribution analysis revealed that the highest expression of foxo1 was in liver. After in vitro analysis, foxo1-specific small interfering RNA (sifoxol) treatment significantly decreased the expressions of cytosolic phosphoenolpyruvate carboxykinase (cpepck) and glucose-6phosphatase1(g6pase1) in primary hepatocytes. Expression of mitochondria] phosphoenolpyruvate carboxykinase (mpepck) was not significantly inhibited. In contrast, the expression of glucose-6-phosphatase2 (g6pase2) increased significantly. After the in vivo study (feeding trial), with the decreased expression of foxo1 in turbot due to high dietary carbohydrate level (21%), the expression of g6pase2 was significantly upregulated. However, the expression of glucokinase (gk) was not changed significantly. These increased the level of blood glucose and hepatic glycogen. In conclusion, data from both in vitro (primary hepatocytes) and in vivo (feeding trial) showed that downregulated foxo1 in turbot could not result in significant depression of gluconeogenesis and activation of glycolysis. This could be one of the reasons why intake of high level of carbohydrate resulted in prolonged hyperglycemia in turbot.
引用
收藏
页码：49 / 56
页数：8
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