Metformin modulates mitochondrial function and mitophagy in peripheral blood mononuclear cells from type 2 diabetic patients

被引：61

作者：

de Maranon, Aranzazu M. ^{[1
]}

Diaz-Pozo, Pedro ^{[1
]}

Canet, Francisco ^{[1
]}

Diaz-Morales, Noelia ^{[1
]}

Abad-Jimenez, Zaida ^{[1
]}

Lopez-Domenech, Sandra ^{[1
]}

Vezza, Teresa ^{[1
]}

Apostolova, Nadezda ^{[2
,3
]}

Morillas, Carlos ^{[1
]}

Rocha, Milagros ^{[1
,2
,5
]}

Victor, Victor M. ^{[1
,2
,4
,5
]}

机构：

[1] Univ Hosp Doctor Peset, Fdn Promot Hlth & Biomed Res Valencian Reg FISABIO, Serv Endocrinol & Nutr, Valencia, Spain

[2] Univ Valencia, CIBERehd Dept Pharmacol, Valencia, Spain

[3] Fdn Promot Hlth & Biomed Res Valencian Reg FISABIO, Valencia, Spain

[4] Univ Valencia, Dept Physiol, Valencia, Spain

[5] Univ Valencia, Univ Hosp Doctor Peset, FISABIO, Avda Gaspar Aguilar 90, Valencia 46017, Spain

来源：

REDOX BIOLOGY | 2022年 / 53卷

关键词：

Metformin; Mitochondria; Mitophagy; Type; 2; diabetes; MEGAMITOCHONDRIA FORMATION; INSULIN-RESISTANCE; OXIDATIVE STRESS; AUTOPHAGY; DYSFUNCTION; METABOLISM; MECHANISM; PROTEIN;

D O I：

10.1016/j.redox.2022.102342

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Type 2 diabetes is a chronic metabolic disease that affects mitochondrial function. In this context, the rescue mechanisms of mitochondrial health, such as mitophagy and mitochondrial biogenesis, are of crucial importance. The gold standard for the treatment of type 2 diabetes is metformin, which has a beneficial impact on the mitochondrial metabolism. In this study, we set out to describe the effect of metformin treatment on mitochondrial function and mitophagy in peripheral blood mononuclear cells (PBMCs) from type 2 diabetic patients. We performed a preliminary cross-sectional observational study complying with CONSORT requirements, for which we recruited 242 subjects, divided into 101 healthy volunteers, 93 metformin-treated type 2 diabetic patients and 48 non-metformin-treated type 2 diabetic patients. Mitochondria from the type 2 diabetic patients not treated with metformin displayed more reactive oxygen species (ROS) than those from healthy or metformintreated subjects. Protein expression of the electron transport chain (ETC) complexes was lower in PBMCs from type 2 diabetic patients without metformin treatment than in those from the other two groups. Mitophagy was altered in type 2 diabetic patients, evident in a decrease in the protein levels of PINK1 and Parkin in parallel to that of the mitochondrial biogenesis protein PGC1 alpha, both of which effects were reversed by metformin. Analysis of AMPK phosphorylation revealed that its activation was decreased in the PBMCs of type 2 diabetic patients, an effect which was reversed, once again, by metformin. In addition, there was an increase in the serum levels of TNF alpha and IL-6 in type 2 diabetic patients and this was reversed with metformin treatment. These results demonstrate that metformin improves mitochondrial function, restores the levels of ETC complexes, and enhances AMPK activation and mitophagy, suggesting beneficial clinical implications in the treatment of type 2 diabetes.

引用

页数：7

共 39 条

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