Genetic requirements for inheritance of RNAi in C-elegans

被引：354

作者：

Grishok, A ^{[1
]}

Tabara, H ^{[1
]}

Mello, CC ^{[1
]}

机构：

[1] Univ Massachusetts, Ctr Canc, Dept Cell Biol, Program Mol Med, Worcester, MA 01605 USA

来源：

SCIENCE | 2000年 / 287卷 / 5462期

关键词：

D O I：

10.1126/science.287.5462.2494

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In Caenorhabditis elegans, the introduction of double-stranded RNA Triggers sequence-specific generic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.

引用

页码：2494 / 2497

页数：4

共 23 条

[1] Double-stranded RNA specifically disrupts gene expression during planarian regeneration [J].

Alvarado, AS ;

Newmark, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) :5049-5054

[2] RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants [J].

Baulcombe, DC .

PLANT MOLECULAR BIOLOGY, 1996, 32 (1-2) :79-88

[3] Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa [J].

Cogoni, C ;

Macino, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) :10233-10238

[4] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[5] A species of small antisense RNA in posttranscriptional gene silencing in plants [J].

Hamilton, AJ ;

Baulcombe, DC .

SCIENCE, 1999, 286 (5441) :950-952

[6] Trans-sensing effects:: The ups and downs of being together [J].

Henikoff, S ;

Comai, L .

CELL, 1998, 93 (03) :329-332

[7] Taming of transposable elements by homology-dependent gene silencing [J].

Jensen, S ;

Gassama, MP ;

Heidmann, T .

NATURE GENETICS, 1999, 21 (02) :209-212

[8] Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway [J].

Kennerdell, JR ;

Carthew, RW .

CELL, 1998, 95 (07) :1017-1026

[9] mut-7 of C-elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD [J].

Ketting, RF ;

Haverkamp, THA ;

van Luenen, HGAM ;

Plasterk, RHA .

CELL, 1999, 99 (02) :133-141

[10] MOLECULAR-CLONING OF THE MUSCLE GENE UNC-22 IN CAENORHABDITIS-ELEGANS BY TC1 TRANSPOSON TAGGING [J].

MOERMAN, DG ;

BENIAN, GM ;

WATERSTON, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2579-2583

← 1 2 3 →