Investigation of sample treatment steps for the analysis of polycyclic aromatic hydrocarbons in ground coffee

被引：39

作者：

Houessou, Justin Koffi ^{[1
]}

Delteil, Corine ^{[1
]}

Camel, Valerie ^{[1
]}

机构：

[1] INRA, INAPG, UMR 214, Ingn Analyt Qual Aliments, F-75231 Paris 05, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 20期

关键词：

coffee; polycyclic aromatic hydrocarbons; pressurized liquid extraction; saponification; solid-phase extraction;

D O I：

10.1021/jf060802z

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Sample treatment procedures were tested for the determination of polycyclic aromatic hydrocarbons (PAHs) in ground coffee. Pressurized liquid extraction (PLE), under different conditions, was combined with several cleanup methods, namely in situ purification, C-18-silica solid-phase extraction (SPE), silica SPE, acid digestion, and alkaline saponification. Soxhlet extraction and direct alkaline saponification were also tested. Best results were obtained using PLE with hexane/acetone 50:50 (v/v) under 150 degrees C. Alkaline saponification followed by cyclohexane extraction and silica SPE was required to eliminate interferent compounds. Finally, 11 PAHs could be quantified in ground coffee with limits of detection in the range of 0.11-0.18 mu g kg(-1). Application to ground Arabica coffee lots from Colombia revealed the presence of several PAHs, giving an overall toxicity equivalence in the range of 0.16-0.87 mu g kg(-1). PAH identification was performed using both high-performance liquid chromatography-diode array detection and gas chromatography coupled to mass spectrometry.

引用

页码：7413 / 7421

页数：9

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