Metabolome analysis based on capillary electrophoresis-mass spectrometry

被引：63

作者：

Hirayama, Akiyoshi ^{[1
]}

Wakayama, Masataka ^{[1
]}

Soga, Tomoyoshi ^{[1
]}

机构：

[1] Keio Univ, Inst Adv Biosci, Tsuruoka, Yamagata 9970052, Japan

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2014年 / 61卷

关键词：

Capillary electrophoresis; CE-MS; Energy metabolism; Mass spectrometry; Metabolite identification; Metabolome analysis; Metabolomics; Sample pretreatment; Separation; Sheathless interface; FUSED-SILICA CAPILLARIES; MS-BASED METABOLOMICS; ELECTROSPRAY-IONIZATION; CE-MS; LC-MS; QUANTITATIVE-ANALYSIS; ZONE-ELECTROPHORESIS; PLANT METABOLOMICS; MIGRATION TIME; PROFILE DATA;

D O I：

10.1016/j.trac.2014.05.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：215 / 222

页数：8

共 76 条

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