The type 1 copper site of pseudoazurin: Axial and rhombic

被引：9

作者：

Gast, Peter ^{[1
]}

Broeren, Freek G. J. ^{[1
]}

Sottini, Silvia ^{[1
]}

Aoki, Risa ^{[2
]}

Takashina, Akiko ^{[2
]}

Yamaguchi, Takahide ^{[2
]}

Kohzuma, Takamitsu ^{[2
]}

Groenen, Edgar J. J. ^{[1
]}

机构：

[1] Leiden Univ, Dept Phys, Huygens Kamerlingh Onnes Lab, NL-2300 RA Leiden, Netherlands

[2] Ibaraki Univ, Inst Appl Beam Sci, Mito, Ibaraki 3108512, Japan

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2014年 / 137卷

关键词：

Blue copper sites; Pseudoazurin; Type; 1; copper; 275 GHz EPR; Absorption spectrum; Plastocyanin; ELECTRON-PARAMAGNETIC-RESONANCE; ATOMIC-RESOLUTION STRUCTURES; ALCALIGENES-FAECALIS S-6; NITRITE REDUCTASE; CRYSTAL-STRUCTURE; ACHROMOBACTER-CYCLOCLASTES; SPECTROSCOPIC PROPERTIES; CONTINUOUS-WAVE; SINGLE-CRYSTAL; PI-INTERACTION;

D O I：

10.1016/j.jinorgbio.2014.03.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on a high-frequency electron-paramagnetic-resonance study of the type 1 copper site of pseudoazurin. The spectra fully resolve the contribution of a nearly axial spectrum besides the rhombic spectrum, which unequivocally proves the existence of two conformations of the copper site. Pseudoazurins have been considered from Achromobacter cycloclastes including eight mutants and from Alcaligenes faecalis. The two conformations are virtually the same for all pseudoazurins, but the rhombic/axial population varies largely, between 91/9 and 33/67. These observations are discussed in relation to optical absorption spectra and X-ray diffraction structures. A similar observation for fern plastocyanin from Dryopteris crassirhizoma suggests that dual conformations of type 1 copper sites are more common. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：57 / 63

页数：7

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