Fluorescence live cell imaging

被引：237

作者：

Ettinger, Andreas ^{[1
]}

Wittmann, Torsten ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Cell & Tissue Biol, San Francisco, CA 94143 USA

来源：

QUANTITATIVE IMAGING IN CELL BIOLOGY | 2014年 / 123卷

关键词：

MONOMERIC RED; DYNAMICS; PROTEIN; MICROTUBULE; DOWNSTREAM; COMPLEX;

D O I：

10.1016/B978-0-12-420138-5.00005-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluorescence microscopy of live cells has become an integral part of modern cell biology. Fluorescent protein (FP) tags, live cell dyes, and other methods to fluorescently label proteins of interest provide a range of tools to investigate virtually any cellular process under the microscope. The two main experimental challenges in collecting meaningful live cell microscopy data are to minimize photodamage while retaining a useful signal-to-noise ratio and to provide a suitable environment for cells or tissues to replicate physiological cell dynamics. This chapter aims to give a general overview on microscope design choices critical for fluorescence live cell imaging that apply to most fluorescence microscopy modalities and on environmental control with a focus on mammalian tissue culture cells. In addition, we provide guidance on how to design and evaluate FP constructs by spinning disk confocal microscopy.

引用

页码：77 / 94

页数：18

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