Minor Contribution of Chimeric Host-HIV Readthrough Transcripts to the Level of HIV Cell-Associated gag RNA

被引:22
作者
Pasternak, Alexander O. [1 ]
DeMaster, Laura K. [2 ]
Kootstra, Neeltje A. [3 ]
Reiss, Peter [4 ,5 ,6 ]
O'Doherty, Una [2 ]
Berkhout, Ben [1 ]
机构
[1] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, Lab Expt Virol, NL-1105 AZ Amsterdam, Netherlands
[2] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA USA
[3] Univ Amsterdam, Acad Med Ctr, Dept Expt Immunol, NL-1105 AZ Amsterdam, Netherlands
[4] Univ Amsterdam, Div Infect Dis, Dept Global Hlth, Amsterdam, Netherlands
[5] Univ Amsterdam, Amsterdam Inst Global Hlth & Dev, Amsterdam, Netherlands
[6] Stichting HIV Monitoring, Amsterdam, Netherlands
关键词
ANTIRETROVIRAL THERAPY; VIRUS; POLYADENYLATION; LATENCY;
D O I
10.1128/JVI.02597-15
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cell-associated HIV unspliced RNA is an important marker of the viral reservoir. HIV gag RNA-specific assays are frequently used to monitor reservoir activation. Because HIV preferentially integrates into actively transcribed genes, some of the transcripts detected by these assays may not represent genuine HIV RNA but rather chimeric host-HIV readthrough transcripts. Here, we demonstrate that in HIV-infected patients on suppressive combination antiretroviral therapy, such host-derived transcripts do not significantly contribute to the HIV gag RNA level.
引用
收藏
页码:1148 / 1151
页数:4
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