Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes

被引：187

作者：

Lorenzi, Hernan ^{[2
]}

Khan, Asis ^{[2
,3
]}

Behnke, Michael S. ^{[2
,4
]}

Namasivayam, Sivaranjani ^{[5
,6
]}

Swapna, Lakshmipuram S. ^{[7
,8
,9
]}

Hadjithomas, Michalis ^{[1
,29
]}

Karamycheva, Svetlana ^{[1
]}

Pinney, Deborah ^{[10
]}

Brunk, Brian P. ^{[10
]}

Ajioka, James W. ^{[11
]}

Ajzenberg, Daniel ^{[12
]}

Boothroyd, John C. ^{[13
]}

Boyle, Jon P. ^{[14
]}

Darde, Marie L. ^{[12
]}

Diaz-Miranda, Maria A. ^{[10
]}

Dubey, Jitender P. ^{[15
]}

Fritz, Heather M. ^{[16
]}

Gennari, Solange M. ^{[17
]}

Gregory, Brian D. ^{[10
]}

Kim, Kami ^{[18
,19
,20
]}

Saeij, Jeroen P. J. ^{[21
]}

Su, Chunlei ^{[22
]}

White, Michael W. ^{[23
,24
]}

Zhu, Xing-Quan ^{[25
]}

Howe, Daniel K. ^{[26
]}

Rosenthal, Benjamin M. ^{[15
]}

Grigg, Michael E. ^{[3
]}

Parkinson, John ^{[7
,8
,9
]}

Liu, Liang ^{[27
,28
]}

Kissinger, Jessica C. ^{[6
,28
]}

Roos, David S. ^{[10
]}

Sibley, L. David ^{[2
]}

机构：

[1] J Craig Venter Inst, Dept Infect Dis, 9704 Med Ctr Dr, Rockville, MD 20850 USA

[2] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63130 USA

[3] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA

[4] Louisiana State Univ, Sch Vet Med, Pathobiol Sci, Baton Rouge, LA 70803 USA

[5] Univ Georgia, Dept Genet, Athens, GA 30602 USA

[6] Univ Georgia, Ctr Trop & Emerging Global Dis, Athens, GA 30602 USA

[7] Hosp Sick Children, Program Mol Struct & Funct, Toronto, ON M5G 1L7, Canada

[8] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[9] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

[10] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

[11] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England

[12] Univ Limoges, Biol Resource Ctr Toxoplasma, Trop Neuroepidemiol Inst Neuroepidemiol & Trop Ne, CHU Limoges,INSERM,UMR S 1094, F-87025 Limoges, France

[13] Stanford Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA

[14] Univ Pittsburgh, Dietrich Sch Arts & Sci, Dept Biol Sci, Pittsburgh, PA 15260 USA

[15] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA

[16] Washington State Univ, Dept Vet Pathol & Microbiol, Coll Vet Med, Pullman, WA 99164 USA

[17] Univ Sao Paulo, Fac Vet Med, Dept Prevent Vet Med & Anim Hlth, BR-05598270 Sao Paulo, Brazil

[18] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA

[19] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA

[20] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 USA

[21] Univ Calif, Dept Pathol Microbiol & Immunol, David, CA 95616 USA

[22] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA

[23] Univ S Florida, Florida Ctr Drug Discovery & Dev CDDI, Dept Mol Med, Tampa, FL 33612 USA

[24] Univ S Florida, Florida Ctr Drug Discovery & Dev CDDI, Dept Global Hlth, Tampa, FL 33612 USA

[25] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China

[26] Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USA

[27] Univ Georgia, Dept Stat, Athens, GA 30602 USA

[28] Univ Georgia, Inst Bioinformat, Athens, GA 30602 USA

[29] DOE Joint Genome Inst, Prokaryot Super Program, Walnut Creek, CA 94598 USA

来源：

NATURE COMMUNICATIONS | 2016年 / 7卷

基金：

美国国家卫生研究院;

关键词：

PLASMODIUM-FALCIPARUM; APICOMPLEXAN PARASITES; ORTHOLOG GROUPS; VIRULENCE; IDENTIFICATION; RECOMBINATION; LINEAGES; SEQUENCE; GENE; ORGANIZATION;

D O I：

10.1038/ncomms10147

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Toxoplasma gondii is among the most prevalent parasites worldwide, infecting many wild and domestic animals and causing zoonotic infections in humans. T. gondii differs substantially in its broad distribution from closely related parasites that typically have narrow, specialized host ranges. To elucidate the genetic basis for these differences, we compared the genomes of 62 globally distributed T. gondii isolates to several closely related coccidian parasites. Our findings reveal that tandem amplification and diversification of secretory pathogenesis determinants is the primary feature that distinguishes the closely related genomes of these biologically diverse parasites. We further show that the unusual population structure of T. gondii is characterized by clade-specific inheritance of large conserved haploblocks that are significantly enriched in tandemly clustered secretory pathogenesis determinants. The shared inheritance of these conserved haploblocks, which show a different ancestry than the genome as a whole, may thus influence transmission, host range and pathogenicity.

引用

页数：13

共 68 条

[31] Modulation of innate immunity by Toxoplasma gondii virulence effectors [J].

Hunter, Christopher A. ;

Sibley, L. David .

NATURE REVIEWS MICROBIOLOGY, 2012, 10 (11) :766-778

[32] Application of phylogenetic networks in evolutionary studies [J].

Huson, DH ;

Bryant, D .

MOLECULAR BIOLOGY AND EVOLUTION, 2006, 23 (02) :254-267

[33] A combined transmembrane topology and signal peptide prediction method [J].

Käll, L ;

Krogh, A ;

Sonnhammer, ELL .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 338 (05) :1027-1036

[34] Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data [J].

Kearse, Matthew ;

Moir, Richard ;

Wilson, Amy ;

Stones-Havas, Steven ;

Cheung, Matthew ;

Sturrock, Shane ;

Buxton, Simon ;

Cooper, Alex ;

Markowitz, Sidney ;

Duran, Chris ;

Thierer, Tobias ;

Ashton, Bruce ;

Meintjes, Peter ;

Drummond, Alexei .

BIOINFORMATICS, 2012, 28 (12) :1647-1649

[35] Recent transcontinental sweeps of Toxoplasma gondii driven by a single monomorphic' chromosome [J].

Khan, A. ;

Fux, B. ;

Su, C. ;

Dubey, J. P. ;

Darde, M. L. ;

Ajioka, J. W. ;

Rosenthal, B. M. ;

Sibley, L. D. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (37) :14872-14877

[36] Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii [J].

Khan, A ;

Taylor, S ;

Su, C ;

Mackey, AJ ;

Boyle, J ;

Cole, R ;

Glover, D ;

Tang, K ;

Paulsen, IT ;

Berriman, M ;

Boothroyd, JC ;

Pfefferkorn, ER ;

Dubey, JP ;

Ajioka, JW ;

Roos, DS ;

Wootton, JC ;

Sibley, LD .

NUCLEIC ACIDS RESEARCH, 2005, 33 (09) :2980-2992

[37] Geographic Separation of Domestic and Wild Strains of Toxoplasma gondii in French Guiana Correlates with a Monomorphic Version of Chromosome1a [J].

Khan, Asis ;

Ajzenberg, Daniel ;

Mercier, Aurelien ;

Demar, Magalie ;

Simon, Stephane ;

Darde, Marie Laure ;

Wang, Qiuling ;

Verma, Shiv Kumar ;

Rosenthal, Benjamin M. ;

Dubey, Jitender P. ;

Sibley, L. David .

PLOS NEGLECTED TROPICAL DISEASES, 2014, 8 (09)

[38] Genetic analyses of atypical Toxoplasma gondii strains reveal a fourth clonal lineage in North America [J].

Khan, Asis ;

Dubey, J. P. ;

Su, Chunlei ;

Ajioka, James W. ;

Rosenthal, Benjamin M. ;

Sibley, L. David .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2011, 41 (06) :645-655

[39]

Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/NMETH.1923, 10.1038/nmeth.1923]

[40] Inference of Population Structure using Dense Haplotype Data [J].

Lawson, Daniel John ;

Hellenthal, Garrett ;

Myers, Simon ;

Falush, Daniel .

PLOS GENETICS, 2012, 8 (01)

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