NAD(+) binding by yeast alcohol dehydrogenase in the presence of pyrazole and a new method for the determination of the enzyme active site concentration

被引：7

作者：

Trivic, S

Zeremski, J

Leskovac, V

机构：

[1] FAC TECHNOL NOVI SAD, YU-21000 NOVI SAD, YUGOSLAVIA

[2] FAC TECHNOL & MET BELGRADE, YU-11000 BELGRADE, YUGOSLAVIA

[3] FAC SCI NOVI SAD, NOVI SAD, YUGOSLAVIA

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 08期

关键词：

Enzyme; Alcohol; Organic Chemistry; Pyrazole; Alcohol Dehydrogenase;

D O I：

10.1023/A:1018308830633

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The pyrazole method of Theorell and Yonetani (Biochem. Z., 338: 573-553, 1963) has been adapted for the determination of the enzyme active site concentration in yeast alcohol dehydrogenase (EC 1.1.1.1). This method cannot be applied indiscriminately to other alcohol dehydrogenases without modification.

引用

页码：809 / 811

页数：3

共 7 条

[1]

Bergmeyer H., 1970, Methoden der Enzymatischen Analyse

[2]

CLELAND WW, 1979, METHOD ENZYMOL, V63, P101

[3]

FRSHT A, 1985, ENZYME STRUCTURE MEC, P159

[4]

HAYES JE, 1954, J BIOL CHEM, V207, P225