Optimized membrane process to increase hemicellulosic ethanol production from pretreated rice straw by recombinant xylose-fermenting Saccharomyces cerevisiae

被引:16
作者
Sasaki, Kengo [1 ]
Tsuge, Yota [1 ]
Sasaki, Daisuke [2 ]
Hasunuma, Tomohisa [1 ]
Sakamoto, Takatoshi [2 ]
Sakihama, Yuri [2 ]
Ogino, Chiaki [2 ]
Kondo, Akihiko [2 ,3 ,4 ]
机构
[1] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Engn, Dept Chem Sci & Engn, Nada Ku, Kobe, Hyogo 6578501, Japan
[3] RIKEN, Biomass Engn Program, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[4] Korea Univ, Coll Life Sci & Biotechnol, Dept Food Biosci & Technol, Seoul 136713, South Korea
关键词
Rice straw; Hemicellulose; Nanofiltration; Ultrafiltration; Ethanol; BIOETHANOL PRODUCTION; FUEL-ETHANOL; WHEAT-STRAW; ACETIC-ACID; NANOFILTRATION; FERMENTATION; HYDROLYSATE; SEPARATION; SUGARS; TECHNOLOGIES;
D O I
10.1016/j.biortech.2014.06.101
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Oligomeric sugars in the liquid fraction of hot water-pretreated rice straw are more amenable to membrane process than monomeric sugars, as lower pressure is required. Following membrane process was employed: nanofiltration (NF) concentration -> (dilution -> NF concentration) x 2 times -> enzymatic hydrolysis (EH) -> ultrafiltration (UF) permeation [Implication: NF for recovery of oligomeric sugars, dilution and NF for removal of low molecular weight fermentation inhibitors, UF for removal of high molecular weight fermentation inhibitors and recovery of monomeric sugars after EH]. This process provided the liquid fraction containing 111.4 g L-1 of sugars, corresponding to 681.0 mM as monomeric sugars, from the original liquid fraction (181.1 mM monomeric sugars). Concentrations of low molecular weight fermentation inhibitors, acetic and formic acids, were decreased to 24% and 48%, respectively. Xylose-fermenting recombinant Saccharomyces cerevisiae produced 34.5 +/- 2.2 g L-1 ethanol from the 0.8 times liquid fraction (76% of theoretical yield). (C) 2014 Published by Elsevier Ltd.
引用
收藏
页码:380 / 386
页数:7
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