Design, synthesis and biological evaluation of a novel Cu2+-selective fluorescence sensor for bio-detection and chelation

被引：19

作者：

Gao, Yu ^{[1
]}

Li, Yazhen ^{[1
]}

Yang, Xiping ^{[1
]}

He, Fangfei ^{[1
]}

Huang, Jiamei ^{[1
]}

Jiang, Minghong ^{[1
]}

Zhou, Zaihui ^{[1
]}

Chen, Haijun ^{[1
]}

机构：

[1] Fuzhou Univ, Coll Chem, Fuzhou 350108, Fujian, Peoples R China

来源：

RSC ADVANCES | 2015年 / 5卷 / 98期

基金：

中国国家自然科学基金;

关键词：

LIVING CELLS; COPPER; CU2+; CHEMOSENSOR; THERAPY; DISEASE; WATER; NANOTHERANOSTICS; CANCER; RATS;

D O I：

10.1039/c5ra12620g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel fluorescence sensor was designed and synthesized for detecting Cu2+ with high sensitivity and selectivity. The sensor showed a large red-shift in UV-vis spectra and obvious decline of fluorescent intensity in fluorescence emission spectra upon the addition of Cu2+. Bio-imaging studies and flow cytometric analysis revealed that this sensor was cell-permeable and could be used for detection of changes of intracellular Cu2+, suggesting the suitability of this sensor for biological application. Cell cytotoxicity studies demonstrated it was capable of chelating excess Cu2+ in vitro to modulate the biological functions of Cu2+ with low cytotoxicity. Therefore, this compound could be a promising fluorescent sensor combining the abilities of either detection or modulation of the biological function of Cu2+ in biological conditions.

引用

页码：80110 / 80117

页数：8

共 28 条

[21] Combinational chelation therapy abrogates lead-induced neurodegeneration in rats [J].

Pachauri, Vidhu ;

Saxena, Geetu ;

Mehta, Ashish ;

Mishra, Deepshikha ;

Flora, Swaran J. S. .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2009, 240 (02) :255-264

[22] An urgent need to reassess the safe levels of copper in the drinking water: Lessons from studies on healthy animals harboring no genetic deficits [J].

Pal, Amit ;

Jayamani, Jayagandan ;

Prasad, Rajendra .

NEUROTOXICOLOGY, 2014, 44 :58-60

[23]

Püsküllü MO, 2013, MINI-REV MED CHEM, V13, P365

[24] BODIPY Fluorescent Chemosensor for Cu2+ Detection and Its Applications in Living Cells: Fast Response and High Sensitivity [J].

Quan, Li ;

Sun, Tingting ;

Lin, Wenhai ;

Guan, Xingang ;

Zheng, Min ;

Xie, Zhigang ;

Jing, Xiabin .

JOURNAL OF FLUORESCENCE, 2014, 24 (03) :841-846

[25] Reevaluation of absolute luminescence quantum yields of standard solutions using a spectrometer with an integrating sphere and a back-thinned CCD detector [J].

Suzuki, Kengo ;

Kobayashi, Atsushi ;

Kaneko, Shigeo ;

Takehira, Kazuyuki ;

Yoshihara, Toshitada ;

Ishida, Hitoshi ;

Shiina, Yoshimi ;

Oishic, Shigero ;

Tobita, Seiji .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2009, 11 (42) :9850-9860

[26] Preparation and sonodynamic activities of water-soluble tetra-α-(3-carboxyphenoxyl) zinc(II) phthalocyanine and its bovine serum albumin conjugate [J].

Xu, He-Nan ;

Chen, Hai-Jun ;

Zheng, Bi-Yuan ;

Zheng, Yun-Quan ;

Ke, Mei-Rong ;

Huang, Jian-Dong .

ULTRASONICS SONOCHEMISTRY, 2015, 22 :125-131

[27] Diethanolamine-Modified Magnetic Fluorescent Fe3O4@ZnS Nanoparticles for Ultrasensitive Detection and Removal of Cu2+ [J].

Yang, Peilun ;

Zhu, Baocun ;

Zhao, Jie ;

Yu, Haiqin ;

Yan, Liangguo ;

Wei, Qin ;

Du, Bin .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2014, 14 (07) :5426-5429

[28] Development of a novel water-soluble magnetic fluorescent nanoparticle for the selective detection and removal of Cu2+ [J].

Zhu, Baocun ;

Yang, Peilun ;

Yu, Haiqin ;

Yan, Liangguo ;

Wei, Qin ;

Du, Bin .

NANOTECHNOLOGY, 2013, 24 (49)

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