Lack of specificity of antibodies directed against human beta-adrenergic receptors

被引：62
作者：
Hamdani, Nazha [1 ]
van der Velden, Jolanda [1 ]
机构：
[1] Vrije Univ Amsterdam Med Ctr, Physiol Lab, Inst Cardiovasc Res, NL-1081 BT Amsterdam, Netherlands
来源：
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | 2009年 / 379卷 / 04期
关键词：
Beta-1; adrenoceptor; Beta-2; Antibody; Selectivity; HUMAN VENTRICULAR MYOCARDIUM; BETA-2-ADRENERGIC RECEPTOR; HEART-FAILURE; DOWN-REGULATION; SUBTYPES; CYCLASE;
D O I：
10.1007/s00210-009-0392-1
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The present study was designed to investigate if antibodies against beta-adrenergic receptors (beta ARs) can be used to determine expression of beta AR in human myocardium. Western blotting was performed to investigate the specificity of antibodies directed against beta(1)AR and beta(2)AR in human left ventricular tissue. A comparison was made between cardiac tissue from patients with idiopathic dilated cardiomyopathy and ischemic heart disease and nonfailing donors. The antibodies directed against beta(1)AR and beta(2)AR recognized several protein bands at different molecular weights. Moreover, both antibodies also recognized multiple proteins in Chinese hamster ovary cells expressing beta(1)AR, beta(2)AR, and even beta(3)AR. beta AR antibodies are not specific and are not suited to study expression of beta AR in human myocardium.
引用
收藏
页码：403 / 407
页数：5
相关论文
共 15 条
[1]   Functional β-adrenergic receptor signalling on nuclear membranes in adult rat and mouse ventricular cardiomyocytes [J].
Boivin, Benoit ;
Lavoie, Catherine ;
Vaniotis, George ;
Baragli, Alessandra ;
Villeneuve, Louis-Robert ;
Ethier, Nathalie ;
Trieu, Phan ;
Allen, Bruce G. ;
Hebert, Terence E. .
CARDIOVASCULAR RESEARCH, 2006, 71 (01) :69-78
[2]  
BRISTOW MR, 1989, MOL PHARMACOL, V35, P295
[3]   DIFFERENCES IN BETA-ADRENERGIC NEUROEFFECTOR MECHANISMS IN ISCHEMIC VERSUS IDIOPATHIC DILATED CARDIOMYOPATHY [J].
BRISTOW, MR ;
ANDERSON, FL ;
PORT, JD ;
SKERL, L ;
HERSHBERGER, RE ;
LARRABEE, P ;
OCONNELL, JB ;
RENLUND, DG ;
VOLKMAN, K ;
MURRAY, J ;
FELDMAN, AM .
CIRCULATION, 1991, 84 (03) :1024-1039
[4]   BETA-1-ADRENERGIC-RECEPTOR AND BETA-2-ADRENERGIC-RECEPTOR SUBPOPULATIONS IN NONFAILING AND FAILING HUMAN VENTRICULAR MYOCARDIUM - COUPLING OF BOTH RECEPTOR SUBTYPES TO MUSCLE-CONTRACTION AND SELECTIVE BETA-1-RECEPTOR DOWN-REGULATION IN HEART-FAILURE- [J].
BRISTOW, MR ;
GINSBURG, R ;
UMANS, V ;
FOWLER, M ;
MINOBE, W ;
RASMUSSEN, R ;
ZERA, P ;
MENLOVE, R ;
SHAH, P ;
JAMIESON, S ;
STINSON, EB .
CIRCULATION RESEARCH, 1986, 59 (03) :297-309
[5]   β-adrenoceptor blocker treatment and the cardiac β-adrenoceptor-G-protein(s)-adenylyl cyclase system in chronic heart failure [J].
Brodde, Otto-Erich .
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2007, 374 (5-6) :361-372
[6]   CLONING OF THE GENE AND CDNA FOR MAMMALIAN BETA-ADRENERGIC-RECEPTOR AND HOMOLOGY WITH RHODOPSIN [J].
DIXON, RAF ;
KOBILKA, BK ;
STRADER, DJ ;
BENOVIC, JL ;
DOHLMAN, HG ;
FRIELLE, T ;
BOLANOWSKI, MA ;
BENNETT, CD ;
RANDS, E ;
DIEHL, RE ;
MUMFORD, RA ;
SLATER, EE ;
SIGAL, IS ;
CARON, MG ;
LEFKOWITZ, RJ ;
STRADER, CD .
NATURE, 1986, 321 (6065) :75-79
[7]   THE SIZE OF THE MAMMALIAN LUNG BETA-2-ADRENERGIC RECEPTOR AS DETERMINED BY TARGET SIZE ANALYSIS AND IMMUNOAFFINITY CHROMATOGRAPHY [J].
FRASER, CM ;
VENTER, JC .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 109 (01) :21-29
[8]  
GEORGE ST, 1986, J BIOL CHEM, V261, P6559
[9]   Calcium cycling in congestive heart failure [J].
Hasenfuss, G ;
Pieske, B .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2002, 34 (08) :951-969
[10]   MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE HUMAN A431 BETA-2-ADRENERGIC RECEPTOR RECOGNIZE 2 MAJOR POLYPEPTIDE-CHAINS [J].
KAVERI, SV ;
CERVANTESOLIVIER, P ;
DELAVIERKLUTCHKO, C ;
STROSBERG, AD .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 167 (03) :449-456
12