Trichosanthin interacts with and enters cells via LDL receptor family members

被引：80

作者：

Chan, WL

Shaw, PC ^{[1
]}

Tam, SC

Jacobsen, C

Gliemann, J

Nielsen, MS

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Dept Physiol, Shatin, Hong Kong, Peoples R China

[3] Univ Aarhus, Dept Med Biochem, DK-8000 Aarhus C, Denmark

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 270卷 / 02期

关键词：

trichosanthin; ribosome-inactivating protein; receptor-associated protein; the alpha(2)-macroglobulin receptor/LDL receptor related protein; choriocarcinoma cells; receptor binding;

D O I：

10.1006/bbrc.2000.2441

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type-I ribosome-inactivating protein trichosan-thin displays selective cytotoxicity, suggesting specific mechanisms for entry into cells, Here we show that trichosanthin binds specifically to the endocytic receptors LRP and megalin, and that binding as well as uptake into cells is inhibited by the receptor-associated protein (RAP). The results suggest that the known abortifacient and renotoxic actions of trichosanthin are caused by LRP-mediated uptake in trophoblasts and megalin-mediated uptake in proximal tubule epithelial cells, respectively, (C) 2000 Academic Press.

引用

页码：453 / 457

页数：5

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