Photometric determination of compounds in paper matrices using digital imaging technique and transmitted light images

Computer-assisted photometric determination of chemical compounds in paper matrices (chromatographic paper circles 10 mm in diameter) is described. A device with a video camera attached to a computer was developed for image density measurements. Matrices containing certified neutral-density glass filters and Congo Red in different concentrations were used. It was shown that the digital recording and computer-assisted photometric analysis of matrix images can be used for determining analyte concentrations in the matrices and assessing the optical homogeneity of these matrices. The advantages and areas of application the method were discussed.