Induction of IκB:: atrial natriuretic peptide as a regulator of the NF-κB pathway

被引：54

作者：

Kiemer, AK ^{[1
]}

Weber, NC ^{[1
]}

Vollmar, AM ^{[1
]}

机构：

[1] Univ Munich, Ctr Drug Res, Dept Pharm, D-81377 Munich, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 295卷 / 05期

关键词：

cardiovascular hormone; natriuretic peptides; cGMP; NPR-A; adhesion molecules; TNF-alpha; endothelial cells; HUVEC;

D O I：

10.1016/S0006-291X(02)00807-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Atrial natriuretic peptide (ANP) was shown to possess anti-inflammatory potential due to its potency to inhibit the production of inflammatory mediators, such as TNF-alpha. The aim of this study was to determine potential effects of ANP on endothelial cells targeted by TNF-alpha. HUVEC were treated with TNF-alpha and expression of adhesion molecules was investigated by FACS and RTPCR. Pre-treatment of cells with ANP (30 min) significantly reduced TNF-alpha-induced cell surface protein and mRNA expression of E-selectin and ICAM-1, whereas it did not influence VCAM-1. ANP reduced TNF-alpha-induced NF-kappaB activity, which was paralleled by a decreased translocation of p65 to nuclei. ANP did not alter TNF-alpha-induced phosphorylation and degradation of IkappaB-alpha, but attenuated degradation of IkappaB-epsilon. Moreover, ANP leads to a transcriptional induction of IkappaB-alpha. The induction of IkappaB by ANP is suggested as a novel mechanism for regulating inflammatory signalling in endothelial cells, leading to reduced TNF-alpha-induced expression of adhesion molecules. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：1068 / 1076

页数：9

共 50 条

[21] The IκB kinase:: A master regulator of NF-κB, innate immunity, and epidermal differentiation
Delhase, M
Karin, M
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1999, 64 : 491 - 503
[22] The noncanonical NF-κB pathway
Sun, Shao-Cong
IMMUNOLOGICAL REVIEWS, 2012, 246 : 125 - 140
[23] The IKK/NF-κB pathway
Senftleben, U
Karin, M
CRITICAL CARE MEDICINE, 2002, 30 (01) : S18 - S26
[24] Porcine arterivirus activates the NF-κB pathway through IκB degradation
Lee, SM
Kleiboeker, SB
VIROLOGY, 2005, 342 (01) : 47 - 59
[25] Activation of NF-κB pathway (NF-κB inducing kinase/NIK, I-κB kinase/Iκ-K, IκBα, NF-κB subunits) and cytoskeletal protein instability in the mucosa of patients with inflammatory bowel disease (IBD)
Banan, A
Shaikh, M
Mutlu, E
Zhang, LJ
Kommanduri, S
Farhadi, A
Keshavarzian, A
GASTROENTEROLOGY, 2004, 126 (04) : A209 - A209
[26] Suppression of NF-κB and NF-κB-Regulated Gene Expression by Apigenin through IκBα and IKK Pathway in TRAMP Mice
Shukla, Sanjeev
Shankar, Eswar
Fu, Pingfu
MacLennan, Gregory T.
Gupta, Sanjay
PLOS ONE, 2015, 10 (09):
[27] A scallop IκB protein involved in innate immunity acts as a key regulator of NF-κB
Chen, Jiwen
Qu, Yifan
Dong, Juan
Xu, Wenwen
Zhao, Yue
Cui, Jie
Yu, Zhengjie
Bao, Zihao
Ma, Jilv
Han, Yijing
Liu, Yaqiong
Huang, Baoyu
Wang, Xiaotong
FISH & SHELLFISH IMMUNOLOGY, 2024, 154
[28] The Nuclear NF-κB Regulator IκBζ: Updates on Its Molecular Functions and Pathophysiological Roles
Yamazaki, Soh
CELLS, 2024, 13 (17)
[29] Targeting the NF-κB pathway as a potential regulator of immune checkpoints in cancer immunotherapy
Nasim Ebrahimi
Al-Hasnawi Rasool Riyadh Abdulwahid
Atena Mansouri
Nasrin Karimi
Rashid Jafardoust Bostani
Sheida Beiranvand
Samaneh Adelian
Roya Khorram
Reza Vafadar
Michael R. Hamblin
Amir Reza Aref
Cellular and Molecular Life Sciences, 2024, 81
[30] Targeting the NF-κB pathway as a potential regulator of immune checkpoints in cancer immunotherapy
Ebrahimi, Nasim
Abdulwahid, Al-Hasnawi Rasool Riyadh
Mansouri, Atena
Karimi, Nasrin
Bostani, Rashid Jafardoust
Beiranvand, Sheida
Adelian, Samaneh
Khorram, Roya
Vafadar, Reza
Hamblin, Michael R.
Aref, Amir Reza
CELLULAR AND MOLECULAR LIFE SCIENCES, 2024, 81 (01)

← 1 2 3 4 5 →