Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-RFLP in Iran

被引：12

作者：

Momeni, Zohreh ^{[1
]}

Sadraei, Javid ^{[1
]}

Kazemi, Bahram ^{[2
]}

Dalimi, Abdolhossein ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Fac Med Sci, Parasitol & Entomol Dept, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran, Iran

来源：

EXPERIMENTAL PARASITOLOGY | 2015年 / 159卷

关键词：

Trichomonas vaginalis; Actin gene; Molecular typing; PCR-RFLP; Karaj city; Iran; CLINICAL PRESENTATION; INFECTIONS; RISK; PREVALENCE; EPIDEMIOLOGY; POLYMORPHISM; CLONING; WOMEN;

D O I：

10.1016/j.exppara.2015.10.011

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral, parasitic sexually transmitted infection in the world. At present, little is known regarding the degree of strain variability of T vaginalis. A classification method for T vaginalis strains would be a useful tool in the study of the epidemiology, drug resistance, pathogenesis and transmission of T. vaginalis. Eight different types of actin genes have been identified by PCR-RFLP in 7: vaginalis; the purpose of this study is to determine the genotypes of this parasite in Karaj city, Iran. Forty-five clinical T. vaginalis isolates from vaginal secretions and urine sediment were collected from Karaj city from 2012 through 2014. DNA was extracted and the actin gene was amplified by nested-PCR; all samples were positive. To determine the genetic differences, sequencing on seven samples was conducted. Then, all PCR products were digested with HindII, Msel, and RsaI restriction enzymes. Of 45 isolates, 23 samples (51.1%) were of actin genotype G, 11 samples (24.4%) of genotype E, six samples (13.3%) of genotype H, three samples (6.6%) of genotype I, and two samples (4.4%) were mixed genotypes of G and E. Genetic diversity of T. vaginalis isolates is notable. The actin genotype G may be the dominant genotype in Karaj city, Iran. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：259 / 263

页数：5

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