Evaluation of Epstein-Barr virus antibodies, anti-VCA avidity by immunofluorescence and immunoblot assays for assessment of Epstein-Barr virus immunologic state

被引：6

作者：

Sener, Asli Gamze ^{[1
]}

Afsar, Ilhan ^{[1
]}

Pinar, Ercan ^{[2
]}

机构：

[1] Ataturk Training & Res Hosp, Microbiol & Clin Microbiol Lab, Izmir, Turkey

[2] Ataturk Training & Res Hosp, Dept Otorhinolaryngol Head & Neck Surg, Izmir, Turkey

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 159卷 / 02期

关键词：

Epstein-Barr virus; Antibody; Avidity; Immunoblot; PRIMARY EBV INFECTION; PREVALENCE; DIAGNOSIS; TESTS;

D O I：

10.1016/j.jviromet.2009.04.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three hundred twenty two serum samples were evaluated by indirect immunofluorescence assay (IFA) and immunoblotting for the assessment of the immunologic status of Epstein-Barr virus (EBV). Serological profiles of 322 serum samples were classified by the IgM and IgG antibodies by the immunofluorescence assay as seronegative, acute infection, reactivation and past infection. Higher rates of seropositivity were determined in serum samples belonging to old age groups. Routine tests used for EBV diagnosis were efficient. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：300 / 302

页数：3

共 16 条

[1]

[Anonymous], 1997, CLIN LAB

[2]

Bauer G, 2001, Clin Lab, V47, P223

[3]

Bauer G, 1995, CLIN LAB, V41, P623

[4] Evaluation of 12 commercial tests for detection of Epstein-Barr virus-specific and heterophile antibodies [J].

Bruu, AL ;

Hjetland, R ;

Holter, E ;

Mortensen, L ;

Natås, O ;

Petterson, W ;

Skar, AG ;

Skarpaas, T ;

Tjade, T ;

Åsjo, B .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2000, 7 (03) :451-456

[5] Evaluation of four commercially available Epstein-Barr virus enzyme immunoassays with an immunofluorescence assay as the reference method [J].

Gärtner, BC ;

Hess, RD ;

Bandt, D ;

Kruse, A ;

Rethwilm, A ;

Roemer, K ;

Mueller-Lantzsch, N .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (01) :78-82

[6] Maintenance of serum immunoglobulin G antibodies to Epstein-Barr virus (EBV) nuclear antigen 2 in healthy individuals from different age groups in a Japanese population with a high childhood incidence of asymptomatic primary EBV infection [J].

Harada, S ;

Kamata, Y ;

Ishii, Y ;

Eda, H ;

Kitamura, R ;

Obayashi, M ;

Ito, S ;

Ban, F ;

Kuranari, J ;

Nakajima, H ;

Kuze, T ;

Hayashi, M ;

Okabe, N ;

Senpuku, H ;

Miyasaka, N ;

Nakamura, Y ;

Kanegane, H ;

Yanagi, K .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2004, 11 (01) :123-130

[7] Routine Epstein-Barr virus diagnostics from the laboratory perspective: Still challenging after 35 years [J].

Hess, RD .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3381-3387

[8] Prevalence of primary versus reactivated Epstein-Barr virus infection in patients with VCA IgG-, VCA IgM- and EBNA-1-antibodies and suspected infectious mononucleosis [J].

Nystad, Tone Wikene ;

Myrmel, Helge .

JOURNAL OF CLINICAL VIROLOGY, 2007, 38 (04) :292-297

[9] Serological and clinical findings in patients with serological evidence of reactivated Epstein-Barr virus infection [J].

Obel, N ;

HoierMadsen, M ;

Kangro, H .

APMIS, 1996, 104 (06) :424-428

[10] RELATIONSHIP BETWEEN ANTIBODY-PRODUCTION TO EPSTEIN-BARR-VIRUS (EBV) EARLY ANTIGENS AND VARIOUS EBV-RELATED DISEASES [J].

OOKA, T ;

DETURENNETESSIER, M ;

STOLZENBERG, MC .

SPRINGER SEMINARS IN IMMUNOPATHOLOGY, 1991, 13 (02) :233-247

← 1 2 →