CrMYB73, a PH-like gene, contributes to citric acid accumulation in citrus fruit

被引：36

作者：

Li, Shao-jia ^{[1
,2
,3
]}

Liu, Xiao-juan ^{[1
]}

Xie, Xiu-lan ^{[1
]}

Sun, Chong-de ^{[1
,2
,3
]}

Grierson, Donald ^{[1
,4
]}

Yin, Xue-ren ^{[1
,2
,3
]}

Chen, Kun-song ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Zhejiang, Peoples R China

[3] Zhejiang Univ, State Agr Minist Lab Hort Plant Growth Dev & Qual, Hangzhou 310058, Zhejiang, Peoples R China

[4] Univ Nottingham, Sch Biosci, Plant & Crop Sci Div, Sutton LE12 5RD, Surrey, England

来源：

SCIENTIA HORTICULTURAE | 2015年 / 197卷

基金：

国家高技术研究发展计划(863计划);

关键词：

Ponkan(Citrus reticulate Blanco); PH gene; MYB transcription factor; Citric acid; TRANSCRIPTION FACTORS; PROTEINS; VACUOLE; ATPASE;

D O I：

10.1016/j.scienta.2015.09.037

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Organic acids strongly influence the organoleptic properties of fruit, and in citrus the major organic acid is citric acid. The pH-associated genes (PH) have been widely studied in various fruit and their expression has been suggested to be linked to citric acid accumulation in citrus fruit, but in planta functional evidence is lacking. A novel PH-like gene, named CrMYB73, was isolated from 'Ponkan' (Citrus reticulate Blanco) fruit, and the amino acid sequence analysis suggested that CrMYB73 was an R2R3 MYB protein. Expression of CrMYB73 was positively correlated with citric acid accumulation and its role was tested directly by overexpression in both wild type and anthocyanin-enriched transgenic Nicotiana tabacum. The results indicated that transient overexpression of CrMYB73 leads to citric acid accumulation. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：212 / 217

页数：6

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