A new method for detecting single nucleotide polymorphism using GFP-display

被引：3

作者：

Aoki, T ^{[1
]}

Satoh, K ^{[1
]}

Imamura, T ^{[1
]}

Watabe, H ^{[1
]}

机构：

[1] Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, Dept Biochem, Ishikari, Hokkaido 0610293, Japan

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 60卷 / 01期

关键词：

green fluorescent protein; GFP-display; single nucleotide polymorphism; single amino acid change; SDS-PAGE;

D O I：

10.1016/j.jbbm.2004.04.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The single nucleotide polymorphism (SNP) of aldehyde dehydrogenase-2 (ALDH2) codon 487, GAA (Glu) or AAA (Lys), was examined using green fluorescent protein (GFP)-display, an electrophoretic detection method for single amino acid changes. Although no shift in migration between the GFP-ALDH (Glu487) and GFP-ALDH (Lys487) fusion proteins was observed on SDS/ urea gel, the two migrated to different positions when tagged with Asp. The SNP analysis was performed with GFP-ALDH-Asp3, and GFP-ALDH-Asp3 constructed from donors having the codon GAA/GAA, GAA/AAA or AAA/AAA was detected as different patterns as expected. GFP-display is potentially a unique method in SNP analysis, which does not require any special equipment or chemicals. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：61 / 67

页数：7

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