Expression and localization of recombinant human B2 receptors in the methylotrophic yeast Pichia pastoris

被引：0

作者：

Yang, G. X. ^{[1
]}

Liu, T. L. ^{[1
]}

Zhang, H. ^{[1
]}

Wu, C. Q. ^{[1
]}

Shen, D. L. ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn,Inst Biodivers Sci, Minist Educ,Key Lab Biodivers Sci & Ecol Engn, Shanghai 200433, Peoples R China

来源：

RUSSIAN JOURNAL OF GENETICS | 2006年 / 42卷 / 07期

关键词：

D O I：

10.1134/S1022795406070040

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The human bradykinin B-2 receptor (B2R) fused with green fluorescent protein (GFP) at the C-terminal has been expressed in the methylotrophic yeast Pichia pastoris. In the expression vector, B2R gene was driven under the highly inducible promoter of alcohol oxidase I gene of R pastoris. By fluorescence activated cell sorting (FACS) analysis and Western blot analysis, it was proved that B2R recombinant receptor proteins were expressed at a high level in the yeast. Furthermore, the transformants of R pastoris were monitored with confocal microscopy, a strong green fluorescence was checked out. The recombinant B2R receptor proteins were mainly located on the plasma membrane proved by immunofluorescence microscopy.

引用

页码：728 / 731

页数：4

共 19 条

[1]

[Anonymous], METHOD ENZYMOL

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