A bio-barcode assay for on-chip attomolar-sensitivity protein detection

被引：158
作者：
Goluch, Edgar D.
Nam, Jwa-Min
Georganopoulou, Dimitra G.
Chiesl, Thomas N.
Shaikh, Kashan A.
Ryu, Kee S.
Barron, Annelise E.
Mirkin, Chad A.
Liu, Chang
机构：
[1] Univ Illinois, Dept Bioengn, Micro & Nanotechnol Lab, Urbana, IL 61801 USA
[2] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA
[3] Northwestern Univ, Inst Nanotechnol, Evanston, IL 60208 USA
[4] Northwestern Univ, Dept Chem Engn, Evanston, IL 60208 USA
来源：
LAB ON A CHIP | 2006年 / 6卷 / 10期
关键词：
D O I：
10.1039/b606294f
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles-a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using I mu L total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.
引用
收藏
页码：1293 / 1299
页数：7
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