In vitro regeneration and transformation of Blackstonia perfoliata

被引：13
作者：
Bijelovic, A
Rosic, N
Miljus-Djukic, J
Ninkovic, S
Grubisic, D
机构：
[1] Fac Biol, Inst Bot, YU-11000 Belgrade, Serbia Monteneg, Serbia
[2] Inst Biol Res Sinisa Stankov, YU-11060 Belgrade, Serbia Monteneg, Serbia
[3] Inst Mol Genet & Genet Engn, YU-11000 Belgrade, Serbia Monteneg, Serbia
来源：
BIOLOGIA PLANTARUM | 2004年 / 48卷 / 03期
关键词：
Agrobacterium rhizogenes; hairy roots; regeneration; root culture;
D O I：
10.1023/B:BIOP.0000041083.41294.31
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In vitro root culture of yellow wort (Blackstonia perfoliata ( L.) Huds.) was initiated on Murashige and Skoog ( MS) medium. In the presence of benzylaminopurine ( BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid ( IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.
引用
收藏
页码：333 / 338
页数：6
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