TNF-alpha downregulates the peroxisome proliferator activated receptor-alpha and the mRNAs encoding peroxisomal proteins in rat liver

被引：87
作者：
Beier, K
Volkl, A
Fahimi, HD
机构：
[1] Inst. fur Anat. und Zellbiologie II, Universität Heidelberg, Im Neuenheimer Feld 307
来源：
FEBS LETTERS | 1997年 / 412卷 / 02期
关键词：
tumor necrosis factor alpha; peroxisome; peroxisome proliferator activated receptor;
D O I：
10.1016/S0014-5793(97)00805-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have studied the effects of TNF-alpha on the mRNAs coding for the peroxisome proliferator activated receptor alpha (PPAR-alpha), and for catalase (Cat), acyl-CoA oxidase (AOX), multifunctional enzyme (PH), and beta-actin in rat liver, Total RNA was isolated from livers of male SD-rats 16 h after administration of a single dose of 25 mu g TNF-alpha and mRNAs were analyzed by a novel dot blot RNase protection assay, The mRNAs for PPAR-alpha and for Cat, AOX and PH were significantly reduced by TNF-treatment, In addition, the level of PPAR-alpha protein was also decreased after TNF, In contrast, the mRNA for beta-actin was markedly increased implying that the effect of TNF on PPAR-alpha and the peroxisomal mRNAs is highly selective, This effect may have important implications in perturbation of the lipid metabolism induced by TNF-alpha. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码：385 / 387
页数：3
相关论文
共 37 条
[31]   CERAMIDE ACTIVATES THE STRESS-ACTIVATED PROTEIN-KINASES [J].
WESTWICK, JK ;
BIELAWSKA, AE ;
DBAIBO, G ;
HANNUN, YA ;
BRENNER, DA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (39) :22689-22692
[32]   EFFECTS OF TNF-ALPHA AND PHORBOL ESTER ON HUMAN SURFACTANT PROTEIN AND MNSOD GENE-TRANSCRIPTION INVITRO [J].
WHITSETT, JA ;
CLARK, JC ;
WISPE, JR ;
PRYHUBER, GS .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (06) :L688-L693
[33]   TUMOR-NECROSIS-FACTOR-ALPHA INHIBITS EXPRESSION OF PULMONARY SURFACTANT PROTEIN [J].
WISPE, JR ;
CLARK, JC ;
WARNER, BB ;
FAJARDO, D ;
HULL, WE ;
HOLTZMAN, RB ;
WHITSETT, JA .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (06) :1954-1960
[34]   Tumor necrosis factor-alpha induces the 85-kDa cytosolic phospholipase A(2) gene expression in human bronchial epithelial cells [J].
Wu, T ;
Ikezono, T ;
Angus, CW ;
Shelhamer, JH .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1996, 1310 (02) :175-184
[35]  
YASMINEH WG, 1991, CANCER RES, V51, P3990
[36]   Sensitive nonradioactive dot blot/ribonuclease protection assay for quantitative determination of mRNA [J].
Zhan, JH ;
Fahimi, HD ;
Voelkl, A .
BIOTECHNIQUES, 1997, 22 (03) :500-&
[37]   Negative regulation of peroxisome proliferator-activated receptor-gamma gene expression contributes to the antiadipogenic effects of tumor necrosis factor-alpha [J].
Zhang, B ;
Berger, J ;
Hu, EI ;
Szalkowski, D ;
WhiteCarrington, S ;
Spiegelman, BM ;
Moller, DE .
MOLECULAR ENDOCRINOLOGY, 1996, 10 (11) :1457-1466
1234