TNF-alpha downregulates the peroxisome proliferator activated receptor-alpha and the mRNAs encoding peroxisomal proteins in rat liver

被引：86
作者：
Beier, K
Volkl, A
Fahimi, HD
机构：
[1] Inst. fur Anat. und Zellbiologie II, Universität Heidelberg, Im Neuenheimer Feld 307
来源：
FEBS LETTERS | 1997年 / 412卷 / 02期
关键词：
tumor necrosis factor alpha; peroxisome; peroxisome proliferator activated receptor;
D O I：
10.1016/S0014-5793(97)00805-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have studied the effects of TNF-alpha on the mRNAs coding for the peroxisome proliferator activated receptor alpha (PPAR-alpha), and for catalase (Cat), acyl-CoA oxidase (AOX), multifunctional enzyme (PH), and beta-actin in rat liver, Total RNA was isolated from livers of male SD-rats 16 h after administration of a single dose of 25 mu g TNF-alpha and mRNAs were analyzed by a novel dot blot RNase protection assay, The mRNAs for PPAR-alpha and for Cat, AOX and PH were significantly reduced by TNF-treatment, In addition, the level of PPAR-alpha protein was also decreased after TNF, In contrast, the mRNA for beta-actin was markedly increased implying that the effect of TNF on PPAR-alpha and the peroxisomal mRNAs is highly selective, This effect may have important implications in perturbation of the lipid metabolism induced by TNF-alpha. (C) 1997 Federation of European Biochemical Societies.
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页码：385 / 387
页数：3
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