Comparison of the Pathogenicity of Classical Swine Fever Virus Subgenotype 2.1c and 2.1d Strains from China

被引：10
作者：
Hao, Genxi [1 ,2 ]
Zhang, Huawei [1 ,2 ]
Chen, Huanchun [1 ,2 ,3 ,4 ]
Qian, Ping [1 ,2 ,3 ,4 ]
Li, Xiangmin [1 ,2 ,3 ,4 ]
机构：
[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Lab Anim Virol, Coll Vet Med, Wuhan 430070, Peoples R China
[3] Minist Agr, Key Lab Dev Vet Diagnost Prod, Wuhan 430070, Peoples R China
[4] Cooperat Innovat Ctr Sustainable Pig Prod, Key Lab Prevent Vet Med Hubei Prov, Wuhan 430070, Peoples R China
来源：
PATHOGENS | 2020年 / 9卷 / 10期
关键词：
classical swine fever virus (CSFV); subgenotype; 2.1c; 2.1d; pathogenicity; China; E2; GLYCOPROTEIN; VIRULENCE DETERMINANT; REPLICATION KINETICS; IDENTIFICATION; REGION;
D O I：
10.3390/pathogens9100821
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Classical swine fever (CSF) caused by classical swine fever virus (CSFV) is a highly contagious and devastating disease. The traditional live attenuated C-strain vaccine is widely used to control disease outbreaks in China. Since 2000, subgenotype 2.1 has become dominant in China. Here, we isolated subgenotype 2.1c and 2.1d strains from CSF-suspected pigs. The genetic variations and pathogenesis of subgenotype 2.1c and 2.1d strains were investigated experimentally. We aimed to evaluate and compare the replication characteristics and clinical signs of subgenotype 2.1c and 2.1d strains with those of the typical highly virulent CSFV SM strain. In PK-15 cells, the three CSFV isolates exhibited similar replication levels but significantly lower replication levels compared with the CSFV SM strain. The experimental animal infection model showed that the pathogenicity of subgenotype 2.1c and 2.1d strains was less than that of the CSFV SM strain. According to the clinical scoring system, subgenotype 2.1c (GDGZ-2019) and 2.1d (HBXY-2019 and GXGG-2019) strains were moderately virulent. This study showed that the pathogenicity of CSFV field strains will aid in the understanding of CSFV biological characteristics and the related epidemiology.
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页码：1 / 11
页数：11
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