Colour modulation of blue anthocyanin-derivatives. Lignosulfonates as a tool to improve the water solubility of natural blue dyes

The impact of a lignosulfonate (calcium) (L) on the chromatic features of two anthocyanin-derived bluish dyes (portisin, P and pyranoanthocyanin dimer, PD) was evaluated in aqueous solutions using UV-Visible spectroscopy. It was demonstrated that the interaction between each dye and this macromolecule at pH 1 occurs by two mechanisms, association and copigmentation that are dependent on L concentration. For instance, at low concentrations of L (up to 0.27 mu M) it is observed the association of dyes promoted by their complexation with lignin (different molecules of dye are bound to the same binding sites of L). At higher concentrations of L (25 mu M), the aggregation of dyes decreases and each molecule is bound to a different site of L resulting in the formation of a charge-transfer complex (copigmentation). Moreover, the titration of both dyes (P and PD) in the presence of L (19 mu M) showed a strong stabilization of their pyranoflavylium cation forms revealed by the considerably higher pK(a1) (6.6 +/- 0.1 and 6.2 +/- 0.1 for P and PD, respectively) in the presence of this macromolecule when compared to the values obtained in the absence (pK(a1) = 4.61 +/- 0.03 and 4.93 +/- 0.04). In addition, the water solubility of the dyes in the absence and presence of L was also evaluated by turbidimetry and the results showed that the water soluble lignosulfonate improved the solubility of the dyes over time.