Using Electrospray Ionisation Mass Spectrometry to Study Non-Covalent Interactions

被引：13
|
作者：
McCullough, Bryan J. [1 ]
Gaskell, Simon J. [1 ]
机构：
[1] Univ Manchester, Manchester Interdisciplinary Bioctr, Michael Barber Ctr Mass Spectrometry, Manchester M1 7DN, Lancs, England
来源：
COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2009年 / 12卷 / 02期
关键词：
PROTEIN-PROTEIN INTERACTIONS; ION MOBILITY SPECTROMETRY; GAS-PHASE CONFORMATIONS; HYDROGEN-EXCHANGE; BINDING INTERACTIONS; TANDEM-MS; COMPLEXES; INTACT; STOICHIOMETRY; INHIBITORS;
D O I：
10.2174/138620709787315463
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The key strengths of electrospray over any other ionisation techniques are its soft nature and its ability to produce multiply charged ions. This combination is ideal for the study of non- covalent interactions. In this review article, we cover the basics of studying non-covalent interactions by mass spectrometry - illustrated with examples from our own and other labs - and discuss the current mass spectrometry based methods used for understanding and characterising noncovalent protein complexes.
引用
收藏
页码：203 / 211
页数：9
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