Caprine herpesvirus-1-specific IgG subclasses in naturally and experimentally infected goats

被引:7
|
作者
Marinaro, Mariarosaria [1 ]
Bellacicco, Anna Lucia [2 ]
Camero, Michele [2 ]
Tarsitano, Elvira [2 ]
Tempesta, Maria [2 ]
Cassone, Antonio [1 ]
Buonavoglia, Canio [2 ]
机构
[1] Ist Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, Italy
[2] Univ Bari, Dept Vet Publ Hlth, Bari, Italy
关键词
CpHV-1; IgG subclass; Viral neutralization; IgG; Goat immunity; HERPES-SIMPLEX-VIRUS; GLUTAMIC-ACID DECARBOXYLASE; HUMAN B-CELLS; INTERFERON-GAMMA; IMMUNOGLOBULIN-G; ANTIBODY; VACCINE; INTERLEUKIN-4; PROTECTION; RESPONSES;
D O I
10.1016/j.vetmic.2009.04.008
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Enzyme-linked immunosorbent assays (ELISAs) were developed to detect caprine herpervirus-1 (CpHV-1)-specific lgG1 and IgG2 in sera from 43 naturally infected goats. The analysis of the IgG subclasses showed a dual pattern of distribution in seropositive goats with a major group of animals (36 out of 43) exhibiting significantly higher levels of IgG2 over lgG1 and a minor group (7 out of 43) possessing equal levels of IgG1 and IgG2. Four goats were experimentally infected with a virulent CpHV-1 Ba.1 strain by the intranasal or the intravaginal route and the kinetics of appearance of CpHV-1 -specific IgG, IgG1 and IgG2 in the serum were studied. Two weeks following infection, both IgG1 and IgG2 levels increased although convalescent sera (i.e., collected 5-8 weeks post-infection) showed a clear prevalence of the IgG2 subclass. To determine the contribution of the different IgG subclasses to herpesvirus immunity, serum neutralization (SN) assays were performed in both naturally and experimentally infected goats. The kinetics of SN showed that neutralization activity was mainly associated to the IgG1 subclass and this was also confirmed in naturally infected goats. The results are discussed from the standpoint that the profile of the IgG subclasses is instrumental to study immune responses to CpHV-1 and that vaccination strategies may benefit from this information. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:266 / 272
页数:7
相关论文
共 23 条
  • [1] Antigen-specific IFN-gamma and IL-4 production in caprine herpesvirus infected goats
    Marinaro, Mariarosaria
    Tempesta, Maria
    Tarsitano, Elvira
    Camero, Michele
    Losurdo, Michele
    Buonavoglia, Canio
    Rezza, Giovanni
    RESEARCH IN VETERINARY SCIENCE, 2012, 93 (02) : 662 - 667
  • [2] Evaluation of canine anti-Leishmania IgG subclasses and their relation with skin signs in naturally infected dogs in the northwest of Iran
    Shabestari Asl, Ali
    Sharifi, Majid
    Mohebali, Mehdi
    Zarei, Zabihollah
    Babaloo, Zohreh
    Akhoundi, Behnaz
    TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2013, 37 (05): : 512 - 515
  • [3] Adaptation of a commercial ELISA to determine the IgG avidity in sheep experimentally and naturally infected with Neospora caninum
    Syed-Hussain, S. S.
    Howe, L.
    Pomroy, W. E.
    West, D. M.
    Smith, S. L.
    Williamson, N. B.
    VETERINARY PARASITOLOGY, 2014, 203 (1-2) : 21 - 28
  • [4] Humoral response (IgG) of goats experimentally infected with Fasciola hepatica against cysteine proteinases of adult fluke
    Ruiz, A
    Molina, JM
    González, J
    Martínez-Moreno, FJ
    Gutiérrez, PN
    Martínez-Moreno, A
    VETERINARY RESEARCH, 2003, 34 (04) : 435 - 443
  • [5] Mumps-specific IgG, IgG subclasses and neutralization titres to the vaccine and outbreak mumps strains differ in vaccinated healthy controls, breakthrough mumps infection cases and naturally infected individuals.
    Foley, Deirdre Jane
    Connell, Anna Rose
    Gonzalez, Gabriel
    Connell, Jeff
    Leahy, Timothy Ronan
    De Gascun, Cillian
    Hassan, Jaythoon
    JOURNAL OF CLINICAL VIROLOGY, 2022, 157
  • [6] Comparison of monoclonal and polyclonal antibodies for the detection of canine IgG1 and IgG2, and associations with infection outcome in Leishmania infantum naturally infected dogs
    Carson, Connor
    Quinnell, Rupert J.
    Day, Michael J.
    Courtenay, Orin
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2010, 133 (2-4) : 264 - 268
  • [7] A live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats
    Thiry, J
    Tempesta, M
    Camero, M
    Tarsitano, E
    Bellacicco, AL
    Thiry, E
    Buonavoglia, C
    VETERINARY MICROBIOLOGY, 2006, 113 (3-4) : 303 - 308
  • [8] IgG1/IgG2 antibody dichotomy in sera of vaccinated or naturally infected dogs with visceral leishmaniosis
    Mendes, CD
    de Souza, EP
    Borja-Cabrera, GP
    Batista, LMM
    dos Santos, MA
    Parra, LE
    Menz, I
    Palatnik, M
    de Souza, CBP
    VACCINE, 2003, 21 (19-20) : 2589 - 2597
  • [9] Specific anti-Neospora caninum IgG1 and IgG2 antibody responses during gestation in naturally infected cattle and their relationship with gamma interferon production
    Almeria, S.
    Nogareda, C.
    Santolaria, P.
    Garcia-Ispierto, I.
    Yaniz, J. L.
    Lopez-Gatius, F.
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2009, 130 (1-2) : 35 - 42
  • [10] Flow Cytometric Detection of Mycobacterium avium subsp paratuberculosis-Specific Antibodies in Experimentally Infected and Naturally Exposed Calves
    Schillinger, S.
    Bridger, P. S.
    Bulun, H.
    Fischer, M.
    Akineden, Oe.
    Seeger, T.
    Barth, S.
    Henrich, M.
    Doll, K.
    Buelte, M.
    Menge, C.
    Bauerfeind, R.
    CLINICAL AND VACCINE IMMUNOLOGY, 2013, 20 (09) : 1457 - 1465