Molecular studies of co-formulated strains of the entomopathogenic fungus, Beauveria bassiana

被引:33
作者
Wang, CS
Li, ZZ
Butt, TM
机构
[1] Univ Coll Swansea, Sch Biol Sci, Swansea SA2 8PP, W Glam, Wales
[2] Anhui Agr Univ, Inst Econ Insect & Fungi, Hefei 230036, Peoples R China
关键词
Beauveria bassiana; pathogenicity; co-formulation; 28S rDNA intron; molecular analyses; parasexuality; strain displacement;
D O I
10.1016/S0022-2011(02)00045-9
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
A 28S rDNA intron was used as a molecular marker to distinguish between two single spore strains of Beauveria bassiana, Bb123 and Bb151. When co-formulated and assayed against larvae of Galleria mellonella these strains exhibited no synergistic increase in virulence, rather Bb123 usually dominated. This study shows that the success of any strain to infect Galleria is dependent on the dose and method of inoculation (injection versus immersion). The result of co-formulated strains grown on solid culture also showed that usually one strain dominated, i.e., strain displacement could happen both in vivo and in vitro. The speed by which one strain was displaced following successive sub-culturing on PDA partly depended on the ratio of Bb151 and Bb123. The co-formulated inoculum. could widen the window over which parent strains would be active on different water activity media. Co-infection did result in heterokaryosis within the Galleria host. Molecular studies also showed that the heterokaryon was not stable and could revert back to the parent strain. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:29 / 34
页数:6
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