In vivo X-ray cine-tomography for tracking morphological dynamics

被引：71

作者：

Rolo, Tomy dos Santos ^{[1
]}

Ershov, Alexey ^{[1
,2
]}

van de Kamp, Thomas ^{[1
]}

Baumbach, Tilo ^{[1
]}

机构：

[1] Karlsruhe Inst Technol, ANKA Inst Photon Sci & Synchrotron Radiat, D-76344 Eggenstein Leopoldshafen, Germany

[2] Natl Res Tomsk Polytech Univ, Dept Gen Phys, Inst Phys & Technol, Tomsk 634050, Russia

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 11期

关键词：

in vivo imaging; motion tracking; screw joint; synchrotron; PHASE-CONTRAST MICROTOMOGRAPHY; RECONSTRUCTION; COLEOPTERA;

D O I：

10.1073/pnas.1308650111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable. We introduce in vivo X-ray cine-tomography as a 4D imaging technique developed to study real-time dynamics in small living organisms with micrometer spatial resolution and sub-second time resolution. The method enables insights into the physiology of small animals by tracking the 4D morphological dynamics of minute anatomical features as demonstrated in this work by the analysis of fast-moving screw-and-nut-type weevil hip joints. The presented method can be applied to a broad range of biological specimens and biotechnological processes.

引用

页码：3921 / 3926

页数：6

共 39 条

[1]

[Anonymous], 2000, GEOMATIC METHOD ANAL, DOI DOI 10.1007/3-540-45597-3_

[2] Imaging applications of synchrotron X-ray phase-contrast microtomography in biological morphology and biomaterials science. 1. General aspects of the technique and its advantages in the analysis of millimetre-sized arthropod structure [J].