Deciphering Hematopoietic Stem Cells in Their Niches: A Critical Appraisal of Genetic Models, Lineage Tracing, and Imaging Strategies

被引：135
作者：
Joseph, Chacko [1 ,2 ]
Quach, Julie M. [1 ]
Walkley, Carl R. [1 ,2 ]
Lane, Steven W. [3 ]
Lo Celso, Cristina [4 ]
Purton, Louise E. [1 ,2 ]
机构：
[1] St Vincents Inst Med Res, Stem Cell Regulat Unit, Fitzroy, Vic 3065, Australia
[2] Univ Melbourne, Dept Med, St Vincents Hosp, Fitzroy, Vic 3065, Australia
[3] Univ Queensland, QIMR Berghofer Med Res Inst, Brisbane, Qld 4029, Australia
[4] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England
来源：
CELL STEM CELL | 2013年 / 13卷 / 05期
基金：
英国生物技术与生命科学研究理事会; 英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词：
LASER-SCANNING CYTOMETRY; SITE-SPECIFIC RECOMBINATION; DIPHTHERIA-TOXIN RECEPTOR; BONE-MARROW NICHE; IN-VIVO; TRANSGENIC MICE; CRE-RECOMBINASE; PROGENITOR CELLS; STEM/PROGENITOR CELLS; OSTEOBLAST LINEAGE;
D O I：
10.1016/j.stem.2013.10.010
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental cells provides extensive flexibility in experimental design, but it can also pose significant challenges due to strain-specific differences in cell specificity. Here we outline various genetic approaches for isolating, detecting, and ablating HSCs and niche components and provide a guide for advantages and caveats to consider. We also discuss opportunities and limitations presented by imaging technologies that allow investigation of HSC behavior in situ.
引用
收藏
页码：520 / 533
页数：14
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