Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR

被引：250

作者：

Verweij, Jaco J. ^{[1
]}

Canales, Marco ^{[2
]}

Polman, Katja ^{[3
]}

Ziem, Juventus ^{[4
]}

Brienen, Eric A. T. ^{[1
]}

Polderman, Anton M. ^{[1
]}

van Lieshout, Lisette ^{[1
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Parasitol, NL-2300 RC Leiden, Netherlands

[2] Univ Peruana Cayetano Heredia, Inst Med Trop Alexander von Humboldt, Lima, Peru

[3] Inst Trop Med, Dept Parasitol, B-2000 Antwerp, Belgium

[4] Univ Dev Studies, Sch Med & Hlth Sci, Tamale, Ghana

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2009年 / 103卷 / 04期

关键词：

Strongyloides stercoralis; Strongyloidiasis; Parasitic intestinal disease; Diagnosis; Baermann sedimentation; Real-time PCR; OESOPHAGOSTOMUM-BIFURCUM; NORTHERN GHANA; ENTAMOEBA-HISTOLYTICA; NECATOR-AMERICANUS; INFECTIONS; QUANTIFICATION; PREVALENCE; HOOKWORM; DNA;

D O I：

10.1016/j.trstmh.2008.12.001

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A real-time PCR method targeting the small subunit of the rRNA gene was developed for the detection of Strongyloides stercoralis DNA in faecal samples, including an internal control to detect inhibition of the amplification process. The assay was performed on a range of well-defined control samples (n=145), known positive faecal samples (n=38) and faecal samples from a region in northern Ghana where S. stercoralis infections are highly endemic (n=212), and achieved 100% specificity and high sensitivity. The use of this assay could facilitate monitoring the prevalence and intensity of S. stercoralis infections during helminth intervention programs. Moreover, the use of this assay in diagnostic laboratories could make the introduction of molecular diagnostics feasible in the routine diagnosis of S. stercoralis infections, with a two-fold increase in the detection rate as compared with the commonly used Baermann sedimentation method. (C) 2008 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

引用

页码：342 / 346

页数：5

共 22 条

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