Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

被引：52

作者：

Robin, Aelig ^{[1
,2
]}

Roberts, Gareth A. ^{[3
]}

Kisch, Johannes ^{[1
,2
]}

Sabbadin, Federico ^{[4
]}

Grogan, Gideon ^{[5
]}

Bruce, Neil ^{[4
]}

Turner, Nicholas J. ^{[1
,2
]}

Flitsch, Sabine L. ^{[1
,2
]}

机构：

[1] Univ Manchester, Sch Chem, Manchester M1 7ND, Lancs, England

[2] Univ Manchester, MIB, Manchester M1 7ND, Lancs, England

[3] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

[4] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England

[5] Univ York, Dept Chem, York YO10 5YW, N Yorkshire, England

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 18期

关键词：

ESCHERICHIA-COLI; RHODOCOCCUS SP; CYTOCHROME P450RHF; SYSTEM; FLAVOCYTOCHROME; EXPRESSION; ROLES;

D O I：

10.1039/b901716j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

引用

页码：2478 / 2480

页数：3

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