Histone posttranslational modifications rather than DNA methylation underlie gene reprogramming in pollination-dependent and pollination-independent fruit set in tomato

被引:28
作者
Hu, Guojian [1 ]
Huang, Baowen [1 ]
Wang, Keke [1 ]
Frasse, Pierre [1 ]
Maza, Elie [1 ]
Djari, Anis [1 ]
Benhamed, Moussa [2 ]
Gallusci, Philippe [3 ]
Li, Zhengguo [4 ]
Zouine, Mohamed [1 ]
Bouzayen, Mondher [1 ,4 ]
机构
[1] Univ Toulouse, INRAe INP Toulouse, Genom & Biotechnol Fruits, UMR990, Ave Agrobiopole,CS 32607, F-31326 Castanet Tolosan, France
[2] Univ Paris Saclay, Inst Plant Sci Paris Saclay, Univ Paris Sud, CNRS,INRA,Univ Evry,Univ Paris Diderot,Sorbonne P, Batiment 630, F-91405 Orsay, France
[3] Univ Bordeaux, Bordeaux Sci Agro, INRA, UMR EGFV, 210 Chemin Leysotte,CS 50008, F-33882 Villenave Dornon, France
[4] Chongqing Univ, Inst Adv Interdisciplinary Studies, Ctr Plant Funct Genom, Chongqing 401331, Peoples R China
关键词
auxin; Cas9; CRISPR; DNA methylation; fruit set; histone posttranslational modification; pollination; Solanum lycopersicum; GENOME-WIDE; TRANSCRIPTIONAL ACTIVATION; REPRODUCTIVE DEVELOPMENT; EPIGENETIC MODIFICATIONS; CHROMATIN; EXPRESSION; GROWTH; AUXIN; RICE; REPRESSION;
D O I
10.1111/nph.16902
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Fruit formation comprises a series of developmental transitions among which the fruit set process is essential in determining crop yield. Yet, our understanding of the epigenetic landscape remodelling associated with the flower-to-fruit transition remains poor. We investigated the epigenetic and transcriptomic reprogramming underlying pollination-dependent and auxin-induced flower-to-fruit transitions in the tomato (Solanum lycopersicum) using combined genomewide transcriptomic profiling, global ChIP-sequencing and whole genomic DNA bisulfite sequencing (WGBS). Variation in the expression of the overwhelming majority of genes was associated with change in histone mark distribution, whereas changes in DNA methylation concerned a minor fraction of differentially expressed genes. Reprogramming of genes involved in processes instrumental to fruit set correlated with their H3K9ac or H3K4me3 marking status but not with changes in cytosine methylation, indicating that histone posttranslational modifications rather than DNA methylation are associated with the remodelling of the epigenetic landscape underpinning the flower-to-fruit transition. Given the prominent role previously assigned to DNA methylation in reprogramming key genes of the transition to ripening, the outcome of the present study supports the idea that the two main developmental transitions in fleshy fruit and the underlying transcriptomic reprogramming are associated with different modes of epigenetic regulations.
引用
收藏
页码:902 / 919
页数:18
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