Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathway

被引:49
作者
Han, Chengzheng [1 ]
Xing, Guozheng [2 ]
Zhang, Mengying [3 ]
Zhong, Min [3 ]
Han, Zhen [4 ]
He, Chiyi [4 ]
Liu, Xiaoping [5 ]
机构
[1] Wannan Med Coll, Affiliated Hosp 1, Clin Integrated Tradit Chinese & Western Med, Wuhu 241001, Anhui, Peoples R China
[2] Anhui Univ Technol, Sch Management Sci & Engn, 59 Hudong Rd, Maanshan 243032, Anhui, Peoples R China
[3] Wannan Med Coll, Affiliated Hosp 1, Dept Cent Lab, Wuhu 241001, Anhui, Peoples R China
[4] Wannan Med Coll, Affiliated Hosp 1, Dept Gastroenterol, Wuhu 241001, Anhui, Peoples R China
[5] Wannan Med Coll, Coll Pharm, Wuhu 241001, Anhui, Peoples R China
关键词
wogonoside; human colon cancer cells; phosphatidylinositol; 3; kinase; RAC-alpha serine/threonine-protein kinase; mechanistic target of rapamycin; p70; S6; ACTIVATION; EXPRESSION; ANGIOGENESIS; BAICALIN; EXTRACT; TARGETS; DEATH;
D O I
10.3892/ol.2018.7852
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Wogonoside, the main effective constituent of traditional Chinese medicine Scutellaria, belongs to the glucuronide family, with various functions, including detoxification, anti-inflammation and nourishing gallbladder, lowering blood pressure, diuresis and anti-allergic reactions. However, the effects of wogonoside on human colon cancer cells remain unclear. The present study aimed to investigate the anticancer effect of wogonoside on human colon cancer cells in vitro and its anticancer mechanisms. The results demonstrated that wogonoside significantly inhibited cell growth, induced apoptosis and mitochondrial-mediated autophagy of colon cancer cells. Furthermore, the results revealed that wogonoside significantly increased caspase-3 and caspase-9 expression levels, induced apoptosis regulator Bax/Bcl-2 and microtubule-associated protein 1A/1B-light chain 3 protein expression, suppressed the phosphatidylinositol 3 kinase (PI3K)/RAC-alpha serine/threonine-protein kinase (Akt)/mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) signaling pathway and induced p62 protein expression in colon cancer cells. In conclusion, these results demonstrated that wogonoside inhibits cell growth and induces mitochondrial mediated autophagy-related apoptosis in human colon cancer cells through modulation of the PI3K/Akt/mTOR/p70S6K signaling pathway.
引用
收藏
页码:4463 / 4470
页数:8
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