Enrichment and identification of differentially expressed genes in hepatocellular carcinoma stem-like cells

被引：2

作者：

Li, Jiang ^{[1
]}

Liu, Kai ^{[2
]}

Sheng, Yuehong ^{[3
]}

Zhang, Qin ^{[1
]}

Chen, Lei ^{[1
]}

Qian, Haihua ^{[1
]}

Wu, Hongping ^{[1
]}

Su, Changqing ^{[1
]}

机构：

[1] Navy Mil Med Univ, Dept Mol Oncol, Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp, 225 Changhai Rd, Shanghai 200438, Peoples R China

[2] Navy Mil Med Univ, Dept Biliary Tract Surg IV, Eastern Hepatobiliary Surg Hosp, Shanghai 200438, Peoples R China

[3] Navy Mil Med Univ, Dept Minimal Invas Therapy, Eastern Hepatobiliary Surg Hosp, Shanghai 200438, Peoples R China

来源：

ONCOLOGY LETTERS | 2020年 / 20卷 / 06期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Hepatocellular carcinoma; cancer stem-like cells; high-throughput screening; differentially expressed genes; signaling pathway; COMPLEMENT FACTOR-I; TARGET PREDICTION; CANCER; SURVIVAL; RNA;

D O I：

10.3892/ol.2020.12162

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cancer stem cells are considered to be tumor-initiating cells. To explain the initiation or progression of hepatocellular carcinoma (HCC), we previously established a culture system that may enrich hepatic cancer stem-like cells (HCSCs). However, the regulatory mechanisms by which HCSCs acquire stem cell properties remain unclear. In the present study, three pairs of HCSCs and case-matched human HCC cells were analyzed by high-throughput screening, and novel biomarkers and pathways for the regulation of HCSCs were identified. The results led to the identification and stratification of 406 differentially expressed genes (DEGs), among which 73 GO terms were found to be significantly associated with DEGs in HCSCs, and only complement and coagulation cascade pathways were identified during the development of HCSCs. By combining the results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, it was revealed that 7 genes were downregulated in the complement and coagulation cascade pathways, and 7 miRNAs were predicted to target several downregulated genes involved in these pathways. The results may contribute toward hepatic cancer stem cell studies and novel drug research for HCC treatment.

引用

页数：8

共 37 条

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