Pulsed Laser Activated Cell Sorting with Three Dimensional Sheathless Inertial Focusing

被引：69
作者：
Chen, Yue [1 ]
Chung, Aram J. [2 ]
Wu, Ting-Hsiang [1 ,3 ]
Teitell, Michael A. [4 ,5 ,6 ,7 ]
Di Carlo, Dino [7 ,8 ]
Chiou, Pei-Yu [1 ,7 ,8 ]
机构：
[1] Univ Calif Los Angeles, Dept Mech & Aerosp Engn, Los Angeles, CA 90095 USA
[2] Rensselaer Polytech Inst, Dept Mech Aerosp & Nucl Engn, Troy, NY 12180 USA
[3] Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Inst Mol Biol, Jonsson Comprehens Canc Ctr, Dept Pathol & Lab Med,Broad Stem Cell Res Ctr, Los Angeles, CA 90095 USA
[5] Univ Calif Los Angeles, Inst Mol Biol, Jonsson Comprehens Canc Ctr, Dept Pediat,Broad Stem Cell Res Ctr, Los Angeles, CA 90095 USA
[6] Univ Calif Los Angeles, Inst Mol Biol, Jonsson Comprehens Canc Ctr, Dept Bioengn,Broad Stem Cell Res Ctr, Los Angeles, CA 90095 USA
[7] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA
[8] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA
来源：
SMALL | 2014年 / 10卷 / 09期
关键词：
cell sorting; inertial focusing; lab-on-a-chip devices; lasers; microfluidics; TOTAL ANALYSIS SYSTEMS; MICROFLUIDIC CHANNEL; MAMMALIAN-CELLS; MICROPARTICLES; SEPARATION;
D O I：
10.1002/smll.201302885
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells with high purity at high-throughput using a single layer PDMS channel that can be easily fabricated at low cost. Sort purity of >90% is achieved at a throughput of 10 000 particles s-1 without severe dilution effects from high volume sheath flows. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1746 / 1751
页数：6
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