Deterrent activity of plant lectins on cowpea weevil Callosobruchus maculatus (F.) oviposition

被引：34

作者：

Sadeghi, Amin

Van Damme, Els J. M.

Peumans, Willy J.

Smagghe, Guy

机构：

[1] Univ Ghent, Lab Agrozool, Dept Crop Protect, Fac Biosci Engn, B-9000 Ghent, Belgium

[2] Univ Ghent, Lab Biochem & Glycobiol, Dept Mol Biotechnol, Fac Biosci Engn, B-9000 Ghent, Belgium

来源：

PHYTOCHEMISTRY | 2006年 / 67卷 / 18期

关键词：

plant lectin; cowpea weevil; callosobruchus maculatus; binary choice assay; oviposition; egg development;

D O I：

10.1016/j.phytochem.2006.06.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of 14 plant lectins was screened in a binary choice bioassay for inhibitory activity on cowpea weevil Callosobruchus maculatus (F.) oviposition. Coating of chickpea seeds (Cicer arietinum L.) with a 0.05% (w/v) solution of plant lectins caused a significant reduction in egg laying. Control experiments with heat inactivated lectin and BSA indicated that the observed deterrent effects are specific and require carbohydrate-binding activity. However, no clear correlation could be established between deterrent activity and sugar-binding specificity/molecular structure of the lectins. Increasing the insect density reduced the inhibitory effect of the lectins confirming that female insects are capable of adjusting their oviposition rates as a function of host availability. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2078 / 2084

页数：7

共 29 条

[1] Chemosensory proteins of Locusta migratoria [J].