Comprehensive Transcriptomic Analysis Reveals Dysregulated Competing Endogenous RNA Network in Endocrine Resistant Breast Cancer Cells

被引：18

作者：

Gao, Liang ^{[1
]}

Shen, Kunwei ^{[2
]}

Yin, Ni ^{[3
]}

Jiang, Min ^{[3
]}

机构：

[1] Soochow Univ, Inst Biol & Med Sci, Suzhou, Peoples R China

[2] Shanghai Jiao Tong Univ, Ruijin Hosp, Comprehens Breast Hlth Ctr, Sch Med, Shanghai, Peoples R China

[3] Soochow Univ, Dept Oncol, Affiliated Hosp 1, Suzhou, Peoples R China

来源：

FRONTIERS IN ONCOLOGY | 2020年 / 10卷

基金：

中国国家自然科学基金;

关键词：

transcriptome profile; endocrine resistance; breast cancer; non-coding RNA; tamoxifen; fulvestrant; TAMOXIFEN RESISTANCE; ESTROGEN-RECEPTOR; DOWN-REGULATION; EXPRESSION; INHIBITION; PATHWAYS;

D O I：

10.3389/fonc.2020.600487

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background Tamoxifen and fulvestrant, both approved for endocrine therapy, have remarkably increased the prognosis of hormone receptor-positive breast cancer patients. However, acquired resistance to endocrine therapy greatly reduces its clinical efficacy. Accumulating evidence suggests a pivotal role of non-coding RNAs (ncRNAs) in breast cancer endocrine resistance, but the specific functions of ncRNAs in tamoxifen and fulvestrant resistance remain largely unknown. Methods Microarray analysis was performed for endocrine therapy sensitive (MCF-7), tamoxifen-resistant (LCC2), and dual tamoxifen and fulvestrant-resistant (LCC9) breast cancer cells. Gene ontology and pathway analysis were conducted for functional prediction of the unannotated differentially expressed ncRNAs. Competing endogenous RNA regulatory networks were constructed. Results We discovered a total of 3,129 long non-coding RNAs (lncRNAs), 13,556 circular RNAs (circRNAs), 132 microRNAs, and 3358 mRNAs that were significantly differentially expressed. We constructed co-expression networks for lncRNA-mRNA, circRNA-mRNA, and microRNA-mRNA. In addition, we established lncRNA-microRNA-mRNA and circRNA-microRNA-mRNA regulatory networks to depict ncRNA crosstalk and transcriptomic regulation of endocrine resistance. Conclusions Our study delineates a comprehensive profiling of ncRNAs in tamoxifen and fulvestrant resistant breast cancer cells, which enriches our understanding of endocrine resistance and sheds new light on identifying novel endocrine resistance biomarkers and potential therapeutic targets to overcome endocrine resistance.

引用

页数：20

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