Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro

被引：73
作者：
Benting, J
Rietveld, A
Ansorge, I
Simons, K
机构：
[1] European Mol Biol Lab, Cell Biol & Biophys Programme, D-69117 Heidelberg, Germany
[2] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany
[3] Univ Heidelberg, Zentrum Mol Biol, D-69120 Heidelberg, Germany
来源：
FEBS LETTERS | 1999年 / 462卷 / 1-2期
关键词：
GPI-anchor; fatty acid; lipid raft;
D O I：
10.1016/S0014-5793(99)01501-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and FLAP purified from FRT cells is raft-associated in vitro when reconstituted into liposomes containing raft lipids. In contrast, the GPI-anchored variant surface glycoprotein from Trypanosoma brucei which contains C14 acyl and alkyl chains shows no significant raft association after reconstitution in vitro. These data indicate that the acyl and alkyl chain composition of GPI-anchors determines raft association. (C) 1999 Federation of European Biochemical Societies.
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收藏
页码：47 / 50
页数：4
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