Protein S100-A7 Derived from Digested Dentin Is a Critical Molecule for Dentin Pulp Regeneration

被引：16

作者：

Komichi, Shungo ^{[1
]}

Takahashi, Yusuke ^{[1
]}

Okamoto, Motoki ^{[1
]}

Ali, Manahil ^{[1
]}

Watanabe, Masakatsu ^{[1
]}

Huang, Hailing ^{[1
]}

Nakai, Takeo ^{[2
]}

Cooper, Paul ^{[3
]}

Hayashi, Mikako ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Dent, Dept Restorat Dent & Endodontol, 1-8 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Osaka Res Inst Ind Sci & Technol, Jyoutou Ku, 1-6-50 Morinomiya, Osaka, Osaka 5368853, Japan

[3] Univ Birmingham, Sch Dent, Oral Biol, Birmingham B15 2TT, W Midlands, England

来源：

CELLS | 2019年 / 8卷 / 09期

基金：

日本学术振兴会;

关键词：

wound healing; pulp capping materials; dentinogenesis; proteomics; GROUP BOX 1; EXTRACELLULAR-MATRIX; PROTEOMIC ANALYSIS; PSORIASIN S100A7; DISTINCT ROLES; TGF-BETA; IN-VITRO; EXPRESSION; SIALOPHOSPHOPROTEIN; CELLS;

D O I：

10.3390/cells8091002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Dentin consists of inorganic hard tissue and organic dentin matrix components (DMCs). Various kinds of bioactive molecules are included in DMCs and some of them can be released after digestion by endogenous matrix metalloproteinases (MMPs) in the caries region. Digested DMCs induced by MMP20 have been reported to promote pulpal wound healing processes, but the released critical molecules responsible for this phenomenon are unclear. Here, we identified protein S100-A7 as a critical molecule for pulpal healing in digested DMCs by comprehensive proteomic approaches and following pulp capping experiments in rat molars. In addition, immunohistochemical results indicated the specific distribution of S100-A7 and receptor for advanced glycation end-products (RAGE) as receptor for S100-A7 in the early stage of the pulpal healing process, and following accumulation of CD146-positive stem cells in wounded pulp. Our findings indicate that protein S100-A7 released from dentin by MMP20 might play a key role in dentin pulp regeneration.

引用

页数：19

共 58 条

[1] A site on laminin α5, AQARSAASKVKVSMKF, induces inflammatory cell production of matrix metalloproteinase-9 and chemotaxis [J].

Adair-Kirk, TL ;

Atkinson, JJ ;

Broekelmann, TJ ;

Doi, M ;

Tryggvason, K ;

Miner, JH ;

Mecham, RP ;

Senior, RM .

JOURNAL OF IMMUNOLOGY, 2003, 171 (01) :398-406

[2] Pulp capping of carious exposures: Treatment outcome after 5 and 10 years: A retrospective study [J].

Barthel, CR ;

Rosenkranz, B ;

Leuenberg, A ;

Roulet, JF .

JOURNAL OF ENDODONTICS, 2000, 26 (09) :525-528

[3] Chemoattractant activity of degradation products of fetal and adult skin extracellular matrix for keratinocyte progenitor cells [J].

Brennan, Ellen P. ;

Tang, Xiao-Han ;

Stewart-Akers, Ann M. ;

Gudas, Lorraine J. ;

Badylak, Stephen F. .

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2008, 2 (08) :491-498

[4]

Cassoni P., 2006, INT J CANCER, V60, P216, DOI [10.1002/ijc.2910600215, DOI 10.1002/IJC.2910600215]

[5] The role of matrix metalloproteinases (MMPs) in human caries [J].

Chaussain-Miller, C ;

Fioretti, F ;

Goldberg, M ;

Menashi, S .

JOURNAL OF DENTAL RESEARCH, 2006, 85 (01) :22-32

[6] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification [J].

Cox, Juergen ;

Mann, Matthias .

NATURE BIOTECHNOLOGY, 2008, 26 (12) :1367-1372

[7] Proteolysis of latent transforming growth factor-β (TGF-β)-binding protein-1 by osteoclasts -: A cellular mechanism for release of TGF-β from bone matrix [J].

Dallas, SL ;

Rosser, JL ;

Mundy, GR ;

Bonewald, LF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (24) :21352-21360

[8] Functions of S100 Proteins [J].

Donato, R. ;

Cannon, B. R. ;

Sorci, G. ;

Riuzzi, F. ;

Hsu, K. ;

Weber, D. J. ;

Geczy, C. L. .

CURRENT MOLECULAR MEDICINE, 2013, 13 (01) :24-57

[9] Proteomic Analysis of Human Tooth Pulp: Proteomics of Human Tooth [J].

Eckhardt, Adam ;

Jagr, Michal ;

Pataridis, Statis ;

Miksik, Ivan .

JOURNAL OF ENDODONTICS, 2014, 40 (12) :1961-1966

[10] S100 proteins expressed in phagocytes: a novel group of damage-associated molecular pattern molecules [J].

Foell, Dirk ;

Wittkowski, Helmut ;

Vogl, Thomas ;

Roth, Johannes .

JOURNAL OF LEUKOCYTE BIOLOGY, 2007, 81 (01) :28-37

← 1 2 3 4 5 6 →