Cationic and anionic reverse micelles as the molecular crowding container for G-quadruplex structure

被引：9

作者：

Ho, Meng-Chieh ^{[1
]}

Chang, Chih-Wei ^{[1
]}

机构：

[1] Natl Changhua Univ Educ, Dept Chem, Changhua 50058, Taiwan

来源：

RSC ADVANCES | 2014年 / 4卷 / 39期

关键词：

INTRAMOLECULAR G-QUADRUPLEX; HUMAN TELOMERIC DNA; WATER POOL; FORMS; STABILITY; PROTEINS; DYNAMICS; SEQUENCE;

D O I：

10.1039/c4ra02141j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we tested the feasibility of using reverse micelles (RMs) as molecular crowding containers for DNA G-quadruplex. The results suggest that the RMs formed by anionic surfactants should be avoided for the molecular crowding studies of G-quadruplex structures. In contrast to the molecular crowding condition created by polyethylene glycol, the human telomeric sequence d[AG(3)(T(2)AG(3))(3)] shows different conformations and drug binding stoichiometry in cationic RMs. Cationic RMs allow the DNA to avoid the dehydration effect caused by polyethylene glycol, and are therefore ideal containers for molecular crowding studies.

引用

页码：20531 / 20534

页数：4

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