Genetic tools for manipulating Acinetobacter baumannii genome: an overview

被引:18
作者
Biswas, Indranil [1 ]
机构
[1] Univ Kansas, Med Ctr, Dept Microbiol Mol Genet & Immunol, Kansas City, KS 66160 USA
关键词
GRAM-NEGATIVE BACTERIA; TRANSPOSON MUTANT LIBRARY; NATURAL TRANSFORMATION; BIOFILM FORMATION; ILLEGITIMATE RECOMBINATION; PSEUDOMONAS-AERUGINOSA; ESCHERICHIA-COLI; VIRULENCE FACTOR; DRY SURFACES; SYSTEM;
D O I
10.1099/jmm.0.000081
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Acinetobacter baumannii is an emerging nosocomial pathogen involved in a variety of infections ranging from minor soft-tissue infections to more severe infections such as ventilator-associated pneumonia and bacteraemia. A. baumannii has become resistant to most of the commonly used antibiotics and multidrug-resistant isolates are becoming a severe problem in the healthcare setting. In the past few years, whole-genome sequences of >200 A. baumannii isolates have been generated. Several methods and molecular tools have been used for genetic manipulation of various Acinetobacter spp. Here, we review recent developments of various genetic tools used for modification of the A. baumannii genome, including various ways to inactivate gene function, chromosomal integration and transposon mutagenesis
引用
收藏
页码:657 / 669
页数:13
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