共 26 条
The Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus
被引:17
作者:

Kohlway, Andrew
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Pirakitikulr, Nathan
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Ding, Steve C.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Yang, Feng
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Luo, Dahai
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Lindenbach, Brett D.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Pyle, Anna M.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Dept Chem, New Haven, CT USA
Yale Univ, Howard Hughes Med Inst, New Haven, CT 06511 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
机构:
[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Yale Univ, Dept Chem, New Haven, CT USA
[4] Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA
[5] Yale Univ, Howard Hughes Med Inst, New Haven, CT 06511 USA
基金:
美国国家卫生研究院;
关键词:
PROTEASE DOMAIN;
RNA REPLICATION;
HELICASE;
TRANSLOCATION;
SYSTEM;
NS4A;
D O I:
10.1128/JVI.00745-14
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.
引用
收藏
页码:10970 / 10974
页数:5
相关论文
共 26 条
[1]
Visualizing ATP-Dependent RNA Translocation by the NS3 Helicase from HCV
[J].
Appleby, Todd C.
;
Anderson, Robert
;
Fedorova, Olga
;
Pyle, Anna M.
;
Wang, Ruth
;
Liu, Xiaohong
;
Brendza, Katherine M.
;
Somoza, John R.
.
JOURNAL OF MOLECULAR BIOLOGY,
2011, 405 (05)
:1139-1153

Appleby, Todd C.
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Anderson, Robert
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Fedorova, Olga
论文数: 0 引用数: 0
h-index: 0
机构:
Howard Hughes Med Inst, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
Yale Univ, Bass Ctr, New Haven, CT 06520 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Pyle, Anna M.
论文数: 0 引用数: 0
h-index: 0
机构:
Howard Hughes Med Inst, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
Yale Univ, Bass Ctr, New Haven, CT 06520 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Wang, Ruth
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Liu, Xiaohong
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Brendza, Katherine M.
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA

Somoza, John R.
论文数: 0 引用数: 0
h-index: 0
机构:
Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA Gilead Sci Inc, Dept Struct Chem, Foster City, CA 94404 USA
[2]
Hepatitis C Viral NS3-4A Protease Activity Is Enhanced by the NS3 Helicase
[J].
Beran, Rudolf K. F.
;
Pyle, Anna Marie
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2008, 283 (44)
:29929-29937

Beran, Rudolf K. F.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Pyle, Anna Marie
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
Yale Univ, Howard Hughes Med Inst, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[3]
The serine protease domain of hepatitis C viral NS3 activates RNA helicase activity by promoting the binding of RNA substrate
[J].
Beran, Rudolf K. F.
;
Serebrov, Victor
;
Pyle, Anna Marie
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2007, 282 (48)
:34913-34920

Beran, Rudolf K. F.
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Serebrov, Victor
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA

Pyle, Anna Marie
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[4]
Unmasking the Active Helicase Conformation of Nonstructural Protein 3 from Hepatitis C Virus
[J].
Ding, Steve C.
;
Kohlway, Andrew S.
;
Pyle, Anna M.
.
JOURNAL OF VIROLOGY,
2011, 85 (09)
:4343-4353

Ding, Steve C.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

Kohlway, Andrew S.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

Pyle, Anna M.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Dept Chem, New Haven, CT 06520 USA
Howard Hughes Med Inst, Chevy Chase, MD 20815 USA Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[5]
BOTH NS3 AND NS4A ARE REQUIRED FOR PROTEOLYTIC PROCESSING OF HEPATITIS-C VIRUS NONSTRUCTURAL PROTEINS
[J].
FAILLA, C
;
TOMEI, L
;
DEFRANCESCO, R
.
JOURNAL OF VIROLOGY,
1994, 68 (06)
:3753-3760

FAILLA, C
论文数: 0 引用数: 0
h-index: 0
机构:
IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY

TOMEI, L
论文数: 0 引用数: 0
h-index: 0
机构:
IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY

DEFRANCESCO, R
论文数: 0 引用数: 0
h-index: 0
机构:
IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY IST RIC BIOL MOLEC P ANGELETTI,I-00040 ROME,ITALY
[6]
EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE
[J].
FUERST, TR
;
NILES, EG
;
STUDIER, FW
;
MOSS, B
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1986, 83 (21)
:8122-8126

FUERST, TR
论文数: 0 引用数: 0
h-index: 0
机构: NIAID,VIRAL DIS LAB,BETHESDA,MD 20892

NILES, EG
论文数: 0 引用数: 0
h-index: 0
机构: NIAID,VIRAL DIS LAB,BETHESDA,MD 20892

STUDIER, FW
论文数: 0 引用数: 0
h-index: 0
机构: NIAID,VIRAL DIS LAB,BETHESDA,MD 20892

MOSS, B
论文数: 0 引用数: 0
h-index: 0
机构: NIAID,VIRAL DIS LAB,BETHESDA,MD 20892
[7]
Hepatitis C Virus RNA Replication and Virus Particle Assembly Require Specific Dimerization of the NS4A Protein Transmembrane Domain
[J].
Kohlway, Andrew
;
Pirakitikulr, Nathan
;
Barrera, Francisco N.
;
Potapova, Olga
;
Engelman, Donald M.
;
Pyle, Anna M.
;
Lindenbach, Brett D.
.
JOURNAL OF VIROLOGY,
2014, 88 (01)
:628-642

Kohlway, Andrew
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Pirakitikulr, Nathan
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Barrera, Francisco N.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Potapova, Olga
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Engelman, Donald M.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Pyle, Anna M.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Howard Hughes Med Inst, New Haven, CT 06511 USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA

Lindenbach, Brett D.
论文数: 0 引用数: 0
h-index: 0
机构:
Yale Univ, Dept Chem, New Haven, CT USA
Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
[8]
Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations
[J].
Krieger, N
;
Lohmann, V
;
Bartenschlager, R
.
JOURNAL OF VIROLOGY,
2001, 75 (10)
:4614-4624

Krieger, N
论文数: 0 引用数: 0
h-index: 0
机构:
Johannes Gutenberg Univ Mainz, Inst Virol, D-55131 Mainz, Germany Johannes Gutenberg Univ Mainz, Inst Virol, D-55131 Mainz, Germany

论文数: 引用数:
h-index:
机构:

Bartenschlager, R
论文数: 0 引用数: 0
h-index: 0
机构:
Johannes Gutenberg Univ Mainz, Inst Virol, D-55131 Mainz, Germany Johannes Gutenberg Univ Mainz, Inst Virol, D-55131 Mainz, Germany
[9]
HCVpro: Hepatitis C virus protein interaction database
[J].
Kwofie, Samuel K.
;
Schaefer, Ulf
;
Sundararajan, Vijayaraghava S.
;
Bajic, Vladimir B.
;
Christoffels, Alan
.
INFECTION GENETICS AND EVOLUTION,
2011, 11 (08)
:1971-1977

Kwofie, Samuel K.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa

Schaefer, Ulf
论文数: 0 引用数: 0
h-index: 0
机构:
King Abdullah Univ Sci & Technol, Computat Biosci Res Ctr CBRC, Thuwal 239556900, Saudi Arabia Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa

Sundararajan, Vijayaraghava S.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa

Bajic, Vladimir B.
论文数: 0 引用数: 0
h-index: 0
机构:
King Abdullah Univ Sci & Technol, Computat Biosci Res Ctr CBRC, Thuwal 239556900, Saudi Arabia Univ Western Cape, S African Natl Bioinformat Inst, ZA-7535 Cape Town, South Africa

论文数: 引用数:
h-index:
机构:
[10]
Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase
[J].
Lam, AMI
;
Frick, DN
.
JOURNAL OF VIROLOGY,
2006, 80 (01)
:404-411

Lam, AMI
论文数: 0 引用数: 0
h-index: 0
机构:
New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA

Frick, DN
论文数: 0 引用数: 0
h-index: 0
机构:
New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA