The Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus

被引:17
作者
Kohlway, Andrew [1 ]
Pirakitikulr, Nathan [2 ]
Ding, Steve C. [1 ]
Yang, Feng [4 ]
Luo, Dahai [2 ]
Lindenbach, Brett D. [4 ]
Pyle, Anna M. [2 ,3 ,5 ]
机构
[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Yale Univ, Dept Chem, New Haven, CT USA
[4] Yale Univ, Dept Microbial Pathogenesis, New Haven, CT USA
[5] Yale Univ, Howard Hughes Med Inst, New Haven, CT 06511 USA
基金
美国国家卫生研究院;
关键词
PROTEASE DOMAIN; RNA REPLICATION; HELICASE; TRANSLOCATION; SYSTEM; NS4A;
D O I
10.1128/JVI.00745-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.
引用
收藏
页码:10970 / 10974
页数:5
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