Epigallocatechin-3-gallate protects against cisplatin-induced nephrotoxicity by inhibiting endoplasmic reticulum stress-induced apoptosis

被引：44
作者：
Chen, Binbin [1 ]
Liu, Guangyi [1 ,2 ,3 ]
Zou, Peimei [1 ]
Li, Xing [1 ]
Hao, Qiufa [1 ]
Jiang, Bei [1 ]
Yang, Xiangdong [1 ]
Hu, Zhao [1 ]
机构：
[1] Shandong Univ, Qi Lu Hosp, Dept Nephrol, Jinan 250012, Shandong, Peoples R China
[2] Shandong Univ, Sch Med, Key Lab Expt Teratol, Minist Educ, Jinan 250013, Shandong, Peoples R China
[3] Shandong Univ, Sch Med, Inst Mol Med & Genet, Jinan 250013, Shandong, Peoples R China
来源：
EXPERIMENTAL BIOLOGY AND MEDICINE | 2015年 / 240卷 / 11期
基金：
中国国家自然科学基金;
关键词：
Cisplatin; apoptosis; endoplasmic reticulum stress; epigallocatechin-3-gallate; nephrotoxicity; GREEN-TEA; OXIDATIVE STRESS; CELLS; ANTIOXIDANT; MECHANISMS; DISEASE; HEALTH; EGCG; RAT; POLYPHENOLS;
D O I：
10.1177/1535370215573394
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Cisplatin (CP)-induced nephrotoxicity hampers its application in clinic. Green tea, particularly its predominant polyphenolic constituent epigallocatechin-3-gallate (EGCG), possesses anti-inflammatory, antioxidant, and anti-apoptotic properties. The present study was designed to investigate the protective effects of EGCG against CP-induced nephrotoxicity in mice. Male C57/BL6 mice in different groups received single injection of CP (20mg/kg) and EGCG (100mg/kg) in various sets and kidney tissues and blood were collected after killing. Then, samples were used for biochemical and immunohistochemical assay. Our results showed EGCG decreased biochemical factors and immunohistochemical damage induced by CP. Besides, expression of phosphorylated-extracellular signal-regulated kinase (p-ERK), glucose-regulated protein 78 (GRP78), caspase-12, and apoptosis of kidney were decreased by EGCG via inhibition of endoplasmic reticulum (ER) stress-induced apoptosis.
引用
收藏
页码：1513 / 1519
页数：7
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